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ACAP2 Recombinant Rabbit Monoclonal Antibody [JE65-03]

Usd: 385

Specification

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Catalog# HA722712

ACAP2 Recombinant Rabbit Monoclonal Antibody [JE65-03]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ACAP2 Recombinant Rabbit Monoclonal Antibody [JE65-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human ACAP2 aa 681-730 / 778.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 88 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, LO2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human lung tissue, mouse lung tissue, rat lung tissue.

Conjugation

unconjugated

Clone Number

JE65-03

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000-1:5,000

Target

Function

Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 is a protein that in humans is encoded by the ACAP2 gene.

Background References

1. Liu X et al. CircRNA ACAP2 induces myocardial apoptosis after myocardial infarction by sponging miR-29. Minerva Med. 2022 Feb

2. Zhang G et al. Circ-ACAP2 facilitates the progression of colorectal cancer through mediating miR-143-3p/FZD4 axis. Eur J Clin Invest. 2021 Dec

Subcellular Location

Endosome membrane.

UNIPROT

SwissProt: Q15057 Human

SwissProt: Q6ZQK5 Mouse

SwissProt: Q5FVC7 Rat

Synonyms

ACAP 2 antibody

ACAP2 antibody

ACAP2_HUMAN antibody

ANK repeat and PH domain-containing protein 2 antibody

Arf GAP with coiled coil ANK repeat and PH domain containing protein 2 antibody

Arf GAP with coiled coil ANK repeat and PH domains 2 antibody

Arf-GAP with coiled-coil antibody

ArfGAP with coiled coil ankyrin repeat and PH domains 2 antibody

CENT B 2 antibody

Centaurin beta2 antibody

Expand

Images

  • Western blot analysis of ACAP2 on different lysates with Rabbit anti-ACAP2 antibody (<a href="/products/HA722712" style="font-weight: bold;text-decoration: underline;">HA722712</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: LO2 cell lysate (20 µg/Lane)<br />Lane 4: Jurkat cell lysate (20 µg/Lane)<br />Lane 5: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 6: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 7: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 88 kDa<br />Observed band size: 88 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722712" style="font-weight: bold;text-decoration: underline;">HA722712</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACAP2 on different lysates with Rabbit anti-ACAP2 antibody (HA722712) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: LO2 cell lysate (20 µg/Lane)
    Lane 4: Jurkat cell lysate (20 µg/Lane)
    Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 6: Mouse liver tissue lysate (40 µg/Lane)
    Lane 7: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 88 kDa
    Observed band size: 88 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722712) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-ACAP2 antibody (<a href="/products/HA722712" style="font-weight: bold;text-decoration: underline;">HA722712</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722712" style="font-weight: bold;text-decoration: underline;">HA722712</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-ACAP2 antibody (HA722712) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722712) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ACAP2 antibody (<a href="/products/HA722712" style="font-weight: bold;text-decoration: underline;">HA722712</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722712" style="font-weight: bold;text-decoration: underline;">HA722712</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ACAP2 antibody (HA722712) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722712) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-ACAP2 antibody (<a href="/products/HA722712" style="font-weight: bold;text-decoration: underline;">HA722712</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722712" style="font-weight: bold;text-decoration: underline;">HA722712</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-ACAP2 antibody (HA722712) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722712) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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