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VSIG4 Recombinant Rabbit Monoclonal Antibody [PSH06-55]

Usd: 385

Specification

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Catalog# HA722718

VSIG4 Recombinant Rabbit Monoclonal Antibody [PSH06-55]

Application

  • WB

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

VSIG4 Recombinant Rabbit Monoclonal Antibody [PSH06-55]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human VSIG4 aa 1-304.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 44 kDa

Positive Control

THP-1 cell lysate, Jurkat cell lysate, Human liver tissue lysate, Human lung tissue lysate, human liver tissue, human lung tissue.

Conjugation

unconjugated

Clone Number

PSH06-55

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

V-set and immunoglobulin domain containing 4 is a protein that in humans is encoded by the VSIG4 gene. This gene encodes a v-set and immunoglobulin-domain containing protein that is structurally related to the B7 family of immune regulatory proteins. The encoded protein may be a negative regulator of T-cell responses. This protein is also a receptor for the complement component 3 fragments C3b and iC3b. Alternate splicing results in multiple transcript variants.

Background References

1. Liu B et al. The biology of VSIG4: Implications for the treatment of immune-mediated inflammatory diseases and cancer. Cancer Lett. 2023 Jan

2. Wang Y et al. Hypoxia Induces M2 Macrophages to Express VSIG4 and Mediate Cardiac Fibrosis After Myocardial Infarction. Theranostics. 2023 Apr

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q9Y279 Human

Synonyms

Complement receptor of the immunoglobulin superfamily antibody

CRIg antibody

Ig superfamily protein antibody

Protein Z39Ig antibody

UNQ317/PRO362 antibody

V set and immunoglobulin domain containing 4 antibody

V-set and immunoglobulin domain-containing protein 4 antibody

VSIG4 antibody

VSIG4_HUMAN antibody

Z39IG antibody

Images

  • Western blot analysis of VSIG4 on different lysates with Rabbit anti-VSIG4 antibody (<a href="/products/HA722718" style="font-weight: bold;text-decoration: underline;">HA722718</a>) at 1/1,000 dilution.<br /><br />Lane 1: THP-1 cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: Human liver tissue lysate (40 µg/Lane)<br />Lane 4: Human lung tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 44 kDa<br />Observed band size: 37-55 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722718" style="font-weight: bold;text-decoration: underline;">HA722718</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of VSIG4 on different lysates with Rabbit anti-VSIG4 antibody (HA722718) at 1/1,000 dilution.

    Lane 1: THP-1 cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: Human liver tissue lysate (40 µg/Lane)
    Lane 4: Human lung tissue lysate (40 µg/Lane)

    Predicted band size: 44 kDa
    Observed band size: 37-55 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722718) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-VSIG4 antibody (<a href="/products/HA722718" style="font-weight: bold;text-decoration: underline;">HA722718</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722718" style="font-weight: bold;text-decoration: underline;">HA722718</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-VSIG4 antibody (HA722718) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722718) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-VSIG4 antibody (<a href="/products/HA722718" style="font-weight: bold;text-decoration: underline;">HA722718</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722718" style="font-weight: bold;text-decoration: underline;">HA722718</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-VSIG4 antibody (HA722718) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722718) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"