PDZK1 Recombinant Rabbit Monoclonal Antibody [JE37-70]
Catalog# HA722729
PDZK1 Recombinant Rabbit Monoclonal Antibody [JE37-70]
-
WB
-
IP
-
Human
-
unconjugated
Overview
Product Name
PDZK1 Recombinant Rabbit Monoclonal Antibody [JE37-70]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human PDZK1 aa 470-519 / 519.
Species Reactivity
Human
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 57 kDa
Positive Control
Caco-2 cell lysate, Human liver tissue lysate.
Conjugation
unconjugated
Clone Number
JE37-70
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IP
-
1-2μg/sample
Target
Function
Na(+)/H(+) exchange regulatory cofactor NHE-RF3 is a protein that in humans is encoded by the PDZK1 gene. This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Background References
1. Pan H et al. PDZK1 upregulates nitric oxide production through the PI3K/ERK2 pathway to inhibit porcine circovirus type 2 replication. Vet Microbiol. 2022 Sep
2. Guo L et al. Mechanism of PDZK1 in Hepatocellular Carcinoma Complicated with Hyperuricemia. J Oncol. 2022 Nov
Subcellular Location
Membrane, Cell membrane.
UNIPROT
Synonyms
1700023D20Rik antibody
2610507N21Rik antibody
4921513F16Rik antibody
AI267131 antibody
AI314638 antibody
AL022680 antibody
C terminal linking and modulating protein antibody
CAP70 antibody
CFTR associated protein of 70 kDa antibody
CFTR associated protein, 70-KD antibody
ExpandImages
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Western blot analysis of PDZK1 on different lysates with Rabbit anti-PDZK1 antibody (HA722729) at 1/1,000 dilution.
Lane 1: Caco-2 cell lysate
Lane 2: Human liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 70 kDa
Exposure time: Lane 1: 16 seconds; Lane 2: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722729) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
PDZK1 was immunoprecipitated from 0.2 mg Caco-2 cell lysate with HA722729 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722729 at 1/500 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Caco-2 cell lysate (input)
Lane 2: HA722729 IP in Caco-2 cell lysate
Lane 3: Rabbit IgG instead of HA722729 in Caco-2 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 44 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"