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cGAS Recombinant Rabbit Monoclonal Antibody [PSH06-78]

Usd: 385

Specification

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Catalog# HA722736

cGAS Recombinant Rabbit Monoclonal Antibody [PSH06-78]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

cGAS Recombinant Rabbit Monoclonal Antibody [PSH06-78]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human cGAS aa 101-522.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 59 kDa

Positive Control

THP-1 cell lysate, HT-29 cell lysate, NK-92 cell lysate, Caco-2 cell lysate, HeLa cell lysate, A431 cell lysate, LoVo cell lysate, PANC-1 cell lysate.

Conjugation

unconjugated

Clone Number

PSH06-78

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Cyclic GMP-AMP synthase (cGAS, cGAMP synthase), belonging to the nucleotidyltransferase family, is a cytosolic DNA sensor that activates a type-I interferon response. It is part of the cGAS-STING DNA sensing pathway. It binds to microbial DNA as well as self DNA that invades the cytoplasm, and catalyzes cGAMP synthesis. cGAMP then functions as a second messenger that binds to and activates the endoplasmic reticulum protein STING to trigger type-I IFNs production. Mice lacking cGAS are more vulnerable to lethal infection by DNA viruses and RNA viruses. In addition, cGAS has been shown to be an innate immune sensor of retroviruses including HIV. The human gene encoding cGAS is MB21D1 on chromosome 6.

Background References

1. Zhang J et al. The role of cGAS-STING signaling in pulmonary fibrosis and its therapeutic potential. Front Immunol. 2023 Oct

2. Wang C et al. Ultrasound-responsive low-dose doxorubicin liposomes trigger mitochondrial DNA release and activate cGAS-STING-mediated antitumour immunity. Nat Commun. 2023 Jun

Subcellular Location

Nucleus, Chromosome, Cell membrane, Cytoplasm, cytosol.

UNIPROT

SwissProt: Q8N884 Human

Synonyms

C6orf150 antibody

cGAMP synthase antibody

cGAS antibody

CGAS_HUMAN antibody

cGMP Synthase antibody

Chromosome 6 open reading frame 150 antibody

Cyclic GMP-AMP synthase antibody

h-cGAS antibody

Hypothetical protein LOC115004 antibody

Mab 21 domain containing 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of cGAS on different lysates with Rabbit anti-cGAS antibody (<a href="/products/HA722736" style="font-weight: bold;text-decoration: underline;">HA722736</a>) at 1/2,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: HT-29 cell lysate<br />Lane 3: NK-92 cell lysate<br />Lane 4: Caco-2 cell lysate<br />Lane 5: HCT 116 cell lysate (low expression)<br />Lane 6: HepG2 cell lysate (low expression)<br />Lane 7: HeLa cell lysate<br />Lane 8: A431 cell lysate<br />Lane 9: LoVo cell lysate<br />Lane 10: PANC-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 59 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722736" style="font-weight: bold;text-decoration: underline;">HA722736</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of cGAS on different lysates with Rabbit anti-cGAS antibody (HA722736) at 1/2,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: HT-29 cell lysate
    Lane 3: NK-92 cell lysate
    Lane 4: Caco-2 cell lysate
    Lane 5: HCT 116 cell lysate (low expression)
    Lane 6: HepG2 cell lysate (low expression)
    Lane 7: HeLa cell lysate
    Lane 8: A431 cell lysate
    Lane 9: LoVo cell lysate
    Lane 10: PANC-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722736) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation