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MED12 Recombinant Rabbit Monoclonal Antibody [PSH06-67]

Usd: 385

Specification

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Catalog# HA722739

MED12 Recombinant Rabbit Monoclonal Antibody [PSH06-67]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

MED12 Recombinant Rabbit Monoclonal Antibody [PSH06-67]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human MED12 aa 1,001-1,350.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 243 kDa

Positive Control

A549 cell lysate, HeLa cell lysate, K-562 cell lysate, LNCaP cell lysate, Jurkat cell lysate, HCT 116 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Rat placenta tissue lysate.

Conjugation

unconjugated

Clone Number

PSH06-67

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae), also known as MED12, is a human gene found on the X chromosome. Mutations in MED12 are responsible for at least two different forms of X-linked dominant mental retardation, Lujan-Fryns syndrome and FG syndrome, as well as instances of prostate cancer. Mutations in MED12 are associated with uterine leiomyomas and breast fibroepithelial tumors (e.g. fibroadenoma and phyllodes tumors).

Background References

1. Plassche SV et al. MED12-Related (Neuro)Developmental Disorders: A Question of Causality. Genes (Basel). 2021 Apr

2. Zuberi A et al. MED12 mutation activates the tryptophan/kynurenine/AHR pathway to promote growth of uterine leiomyomas. JCI Insight. 2023 Sep

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q93074 Human

SwissProt: A2AGH6 Mouse

Entrez Gene: 679693 Rat

Synonyms

Activator recruited cofactor 240 kDa component antibody

Activator-recruited cofactor 240 kDa component antibody

ARC240 antibody

CAG repeat protein 45 antibody

CAGH45 antibody

HOPA antibody

KIAA0192 antibody

MED12 antibody

MED12_HUMAN antibody

Mediator complex subunit 12 antibody

Expand

Images

  • Western blot analysis of MED12 on different lysates with Rabbit anti-MED12 antibody (<a href="/products/HA722739" style="font-weight: bold;text-decoration: underline;">HA722739</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: LNCaP cell lysate<br />Lane 5: Jurkat cell lysate<br />Lane 6: HCT 116 cell lysate<br />Lane 7: Caco-2 cell lysate<br />Lane 8: NIH/3T3 cell lysate<br />Lane 9: C2C12 cell lysate<br />Lane 10: PC-12 cell lysate<br />Lane 11: Rat placenta tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 243 kDa<br />Observed band size: 250 kDa<br /><br />Exposure time: 1 minutes 50 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722739" style="font-weight: bold;text-decoration: underline;">HA722739</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MED12 on different lysates with Rabbit anti-MED12 antibody (HA722739) at 1/1,000 dilution.

    Lane 1: A549 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: LNCaP cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: HCT 116 cell lysate
    Lane 7: Caco-2 cell lysate
    Lane 8: NIH/3T3 cell lysate
    Lane 9: C2C12 cell lysate
    Lane 10: PC-12 cell lysate
    Lane 11: Rat placenta tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 243 kDa
    Observed band size: 250 kDa

    Exposure time: 1 minutes 50 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722739) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"