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CETP Recombinant Rabbit Monoclonal Antibody [JE02-61]

Usd: 385

Specification

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Catalog# HA722752

CETP Recombinant Rabbit Monoclonal Antibody [JE02-61]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CETP Recombinant Rabbit Monoclonal Antibody [JE02-61]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human CETP aa 251-300 / 493.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 55 kDa

Positive Control

Human liver tissue lysate, Mouse liver tissue lysate, Human plasma lysate, Mouse plasma lysate, Rat plasma lysate, human liver tissue.

Conjugation

unconjugated

Clone Number

JE02-61

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Cholesteryl ester transfer protein (CETP), also called plasma lipid transfer protein, is a plasma protein that facilitates the transport of cholesteryl esters and triglycerides between the lipoproteins. It collects triglycerides from very-low-density (VLDL) or Chylomicrons and exchanges them for cholesteryl esters from high-density lipoproteins (HDL), and vice versa. Most of the time, however, CETP does a heteroexchange, trading a triglyceride for a cholesteryl ester or a cholesteryl ester for a triglyceride.

Background References

1. Nicholls SJ et al. CETP Inhibitors: Should We Continue to Pursue This Pathway? Curr Atheroscler Rep. 2022 Dec

2. Cupido AJ et al. Joint Genetic Inhibition of PCSK9 and CETP and the Association With Coronary Artery Disease: A Factorial Mendelian Randomization Study. JAMA Cardiol. 2022 Sep

Subcellular Location

Secreted.

UNIPROT

SwissProt: P11597 Human

Synonyms

BPIFF antibody

CETP antibody

CETP_HUMAN antibody

Cholesteryl ester transfer protein antibody

Cholesteryl ester transfer protein plasma antibody

HDLCQ10 antibody

Lipid transfer protein I antibody

Images

  • Western blot analysis of CETP on different lysates with Rabbit anti-CETP antibody (<a href="/products/HA722752" style="font-weight: bold;text-decoration: underline;">HA722752</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human liver tissue lysate<br />Lane 2: Mouse liver tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 55 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722752" style="font-weight: bold;text-decoration: underline;">HA722752</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CETP on different lysates with Rabbit anti-CETP antibody (HA722752) at 1/1,000 dilution.

    Lane 1: Human liver tissue lysate
    Lane 2: Mouse liver tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 55 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of CETP on different lysates with Rabbit anti-CETP antibody (<a href="/products/HA722752" style="font-weight: bold;text-decoration: underline;">HA722752</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human plasma lysate<br />Lane 2: Mouse plasma lysate<br />Lane 3: Rat plasma lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 55 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722752" style="font-weight: bold;text-decoration: underline;">HA722752</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CETP on different lysates with Rabbit anti-CETP antibody (HA722752) at 1/1,000 dilution.

    Lane 1: Human plasma lysate
    Lane 2: Mouse plasma lysate
    Lane 3: Rat plasma lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 55 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CETP antibody (<a href="/products/HA722752" style="font-weight: bold;text-decoration: underline;">HA722752</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722752" style="font-weight: bold;text-decoration: underline;">HA722752</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CETP antibody (HA722752) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722752) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"