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Hex Recombinant Rabbit Monoclonal Antibody [JE37-45]

Usd: 385

Specification

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Catalog# HA722753

Hex Recombinant Rabbit Monoclonal Antibody [JE37-45]

Application

  • WB

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Hex Recombinant Rabbit Monoclonal Antibody [JE37-45]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Hex aa 91-240 / 270.

Species Reactivity

Human

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 30 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, MCF7 cell lysate, HT-29 cell lysate.

Conjugation

unconjugated

Clone Number

JE37-45

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Hematopoietically-expressed homeobox protein HHEX is a protein that in humans is encoded by the HHEX gene and also known as Proline Rich Homeodomain protein PRH. This gene encodes a member of the homeobox family of transcription factors, many of which are involved in developmental processes. Expression in specific hematopoietic lineages suggests that this protein may play a role in hematopoietic differentiation but the expression of this protein is not limited to hematopoietic cells.

Background References

1. Guo Y et al. CK2-induced cooperation of HHEX with the YAP-TEAD4 complex promotes colorectal tumorigenesis. Nat Commun. 2022 Aug

2. Xu Z et al. HHEX suppresses advanced thyroid cancer by interacting with TLE3. Mol Cell Endocrinol. 2023 Aug

Subcellular Location

Nucleus, nuclear body, Cytoplasm.

UNIPROT

SwissProt: Q03014 Human

Synonyms

Hematopoietically expressed homeobox antibody

Hematopoietically-expressed homeobox protein HHEX antibody

HEX antibody

HHEX antibody

HHEX_HUMAN antibody

HMPH antibody

Homeobox hematopoietically expressed antibody

Homeobox protein HEX antibody

Homeobox protein PRH antibody

HOX11L PEN antibody

Expand

Images

  • Western blot analysis of Hex on different lysates with Rabbit anti-Hex antibody (<a href="/products/HA722753" style="font-weight: bold;text-decoration: underline;">HA722753</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: HT-29 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 30 kDa<br />Observed band size: 32 kDa<br /><br />Exposure time: 46 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722753" style="font-weight: bold;text-decoration: underline;">HA722753</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Hex on different lysates with Rabbit anti-Hex antibody (HA722753) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: HT-29 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 30 kDa
    Observed band size: 32 kDa

    Exposure time: 46 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722753) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Hex was immunoprecipitated from 0.2 mg MCF7 cell lysate with <a href="/products/HA722753" style="font-weight: bold;text-decoration: underline;">HA722753</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722753" style="font-weight: bold;text-decoration: underline;">HA722753</a> at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (<a href="/products/M1003-7" style="font-weight: bold;text-decoration: underline;">M1003-7</a>) at 1/100,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: MCF7 cell lysate (input)<br />Lane 2: <a href="/products/HA722753" style="font-weight: bold;text-decoration: underline;">HA722753</a> IP in MCF7 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722753" style="font-weight: bold;text-decoration: underline;">HA722753</a> in MCF7 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 8 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Hex was immunoprecipitated from 0.2 mg MCF7 cell lysate with HA722753 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722753 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/100,000 dilution was used for 1 hour at room temperature.

    Lane 1: MCF7 cell lysate (input)
    Lane 2: HA722753 IP in MCF7 cell lysate
    Lane 3: Rabbit IgG instead of HA722753 in MCF7 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 8 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"