Phospho-Met (Y1234/Y1235) Recombinant Rabbit Monoclonal Antibody [PSH06-90]
Catalog# HA722765
Phospho-Met (Y1234/Y1235) Recombinant Rabbit Monoclonal Antibody [PSH06-90]
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WB
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Human
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HA751114
不含抗保成分
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unconjugated
Overview
Product Name
Phospho-Met (Y1234/Y1235) Recombinant Rabbit Monoclonal Antibody [PSH06-90]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Tyr1234/Tyr1235 of human Met aa 1,201-1,250.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 156 kDa
Positive Control
HeLa cell lysate, HeLa starved for 16 hours then treated with 20ng/mL HGF for 10 minutes cell lysate.
Conjugation
unconjugated
Clone Number
PSH06-90
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Hepatocyte growth factor receptor (HGF receptor) is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGF receptor is a single pass tyrosine kinase receptor essential for embryonic development, organogenesis and wound healing. Hepatocyte growth factor/Scatter Factor (HGF/SF) and its splicing isoform (NK1, NK2) are the only known ligands of the HGF receptor. MET is normally expressed by cells of epithelial origin, while expression of HGF/SF is restricted to cells of mesenchymal origin. When HGF/SF binds its cognate receptor MET it induces its dimerization through a not yet completely understood mechanism leading to its activation.
Background References
1. Remon J et al. MET alterations in NSCLC-Current Perspectives and Future Challenges. J Thorac Oncol. 2023 Apr
2. Sakamoto M et al. MET alterations in advanced non-small cell lung cancer. Lung Cancer. 2023 Apr
Subcellular Location
Membrane; Secreted.
UNIPROT
Synonyms
AUTS9 antibody
c met antibody
D249 antibody
Hepatocyte growth factor receptor antibody
HGF receptor antibody
HGF/SF receptor antibody
HGFR antibody
MET antibody
Met proto oncogene antibody
Met proto oncogene tyrosine kinase antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-Met (Y1234/Y1235) on different lysates with Rabbit anti-Phospho-Met (Y1234/Y1235) antibody (HA722765) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa starved for 16 hours then treated with 20ng/mL HGF for 10 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 156 kDa
Observed band size: 145 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Phospho-Met (Y1349) Recombinant Rabbit Monoclonal Antibody [JE51-68]
Application: WB,IHC-P
Reactivity: Human,Rat
Conjugate: unconjugated
