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cIAP2 Recombinant Rabbit Monoclonal Antibody [PSH06-92]

Usd: 385

Specification

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Catalog# HA722767

cIAP2 Recombinant Rabbit Monoclonal Antibody [PSH06-92]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

cIAP2 Recombinant Rabbit Monoclonal Antibody [PSH06-92]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human cIAP2 aa 205-604.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 68 kDa

Positive Control

Raji cell lysate, Daudi cell lysate, Ramos cell lysate, NCI-H1299 cell lysate.

Conjugation

unconjugated

Clone Number

PSH06-92

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Baculoviral IAP repeat-containing protein3 (also known as cIAP2) is a protein that in humans is encoded by the BIRC3 gene. cIAP2 is a member of the inhibitor of apoptosis family that inhibit apoptosis by interfering with the activation of caspases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. The cIAP2 protein contains three BIR domains, a UBA domain, a CARD domain and a RING finger domain. Transcript variants encoding the same isoform have been identified.

Background References

1. Glasheen MQ et al. Targeting Upregulated cIAP2 in SOX10-Deficient Drug Tolerant Melanoma. Mol Cancer Ther. 2023 Sep

2. Ding Z et al. cIAP2 expression and clinical significance in pigmented villonodular synovitis. J Mol Histol. 2021 Apr

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q13489 Human

Synonyms

AIP 1 antibody

AIP1 antibody

API 2 antibody

API2 antibody

Apoptosis inhibitor 2 antibody

Baculoviral IAP repeat containing 3 antibody

Baculoviral IAP repeat containing protein 3 antibody

Baculoviral IAP repeat-containing protein 3 antibody

BIRC 3 antibody

BIRC3 antibody

Expand

Images

  • Western blot analysis of cIAP2 on different lysates with Rabbit anti-cIAP2 antibody (<a href="/products/HA722767" style="font-weight: bold;text-decoration: underline;">HA722767</a>) at 1/2,000 dilution.<br /><br />Lane 1: Raji cell lysate<br />Lane 2: Daudi cell lysate<br />Lane 3: Ramos cell lysate<br />Lane 4: NCI-H1299 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 68 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722767" style="font-weight: bold;text-decoration: underline;">HA722767</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of cIAP2 on different lysates with Rabbit anti-cIAP2 antibody (HA722767) at 1/2,000 dilution.

    Lane 1: Raji cell lysate
    Lane 2: Daudi cell lysate
    Lane 3: Ramos cell lysate
    Lane 4: NCI-H1299 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 68 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722767) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"