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FRA1 Recombinant Rabbit Monoclonal Antibody [PSH06-95]

Usd: 385

Specification

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Catalog# HA722771

FRA1 Recombinant Rabbit Monoclonal Antibody [PSH06-95]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

FRA1 Recombinant Rabbit Monoclonal Antibody [PSH06-95]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human FRA1 aa 1-271.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 29 kDa

Positive Control

HeLa starved for 24 hours then add 100ng/mL TPA for 4 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH06-95

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Fos-related antigen 1 (FRA1) is a protein that in humans is encoded by the FOSL1 gene. The Fos gene family consists of 4 members: c-Fos, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.

Background References

1. Manetsch P et al. PARP7-mediated ADP-ribosylation of FRA1 promotes cancer cell growth by repressing IRF1- and IRF3-dependent apoptosis. Proc Natl Acad Sci U S A. 2023 Dec

2. Jiang X et al. Expression and function of FRA1 protein in tumors. Mol Biol Rep. 2020 Jan

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P15407 Human

Synonyms

FOS L1 antibody

FOS like antigen 1 antibody

Fos related antigen 1 antibody

Fos-related antigen 1 antibody

FOSL 1 antibody

FOSL1 antibody

FOSL1 protein antibody

FOSL1_HUMAN antibody

FRA 1 antibody

FRA-1 antibody

Images

  • Western blot analysis of FRA1 on different lysates with Rabbit anti-FRA1 antibody (<a href="/products/HA722771" style="font-weight: bold;text-decoration: underline;">HA722771</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa starved for 24 hours then add 100ng/mL TPA for 4 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 29 kDa<br />Observed band size: 35-40 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722771" style="font-weight: bold;text-decoration: underline;">HA722771</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FRA1 on different lysates with Rabbit anti-FRA1 antibody (HA722771) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa starved for 24 hours then add 100ng/mL TPA for 4 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 29 kDa
    Observed band size: 35-40 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722771) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"