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Aquaporin 5 Recombinant Rabbit Monoclonal Antibody [JE05-51]

Usd: 385

Specification

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Catalog# HA722781

Aquaporin 5 Recombinant Rabbit Monoclonal Antibody [JE05-51]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • mIHC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Aquaporin 5 Recombinant Rabbit Monoclonal Antibody [JE05-51]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Aquaporin 5 aa 225-265 / 265.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Tissue, mIHC

Molecular Weight

Predicted band size: 28 kDa

Positive Control

Human lung tissue lysate, Mouse lung tissue lysate, human stomach tissue, mouse lung tissue.

Conjugation

unconjugated

Clone Number

JE05-51

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:500

  • mIHC

  • 1:1,000

Target

Function

Aquaporin-5 (AQP-5) is a protein that in humans is encoded by the AQP5 gene. Aquaporin-5 (AQP-5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin-5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13.

Background References

1. D\'Agostino C et al. Aquaporin-5 Dynamic Regulation. Int J Mol Sci. 2023 Jan

2. Bystrup M et al. Aquaporin-5 in breast cancer. APMIS. 2022 May

Subcellular Location

Apical cell membrane, Cell membrane, Cytoplasmic vesicle membrane.

UNIPROT

SwissProt: P55064 Human

SwissProt: Q9WTY4 Mouse

Synonyms

AQP 5 antibody

AQP-5 antibody

AQP5 antibody

AQP5_HUMAN antibody

Aquaporin 5 antibody

Aquaporin-5 antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Aquaporin 5 on different lysates with Rabbit anti-Aquaporin 5 antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human lung tissue lysate<br />Lane 2: Human liver tissue lysate (negative)<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 28 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Aquaporin 5 on different lysates with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/1,000 dilution.

    Lane 1: Human lung tissue lysate
    Lane 2: Human liver tissue lysate (negative)

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 28 kDa
    Observed band size: 25 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Aquaporin 5 on different lysates with Rabbit anti-Aquaporin 5 antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse lung tissue lysate<br />Lane 2: Mouse kidney tissue lysate (negative)<br />Lane 3: Mouse liver tissue lysate (negative)<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 28 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Aquaporin 5 on different lysates with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/2,000 dilution.

    Lane 1: Mouse lung tissue lysate
    Lane 2: Mouse kidney tissue lysate (negative)
    Lane 3: Mouse liver tissue lysate (negative)

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 28 kDa
    Observed band size: 25 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722781) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescence analysis of paraffin-embedded human stomach tissue labeling Aquaporin 5 with Rabbit anti-Aquaporin 5 antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human stomach tissue labeling Aquaporin 5 with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722781, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Aquaporin 5 antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722781) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Aquaporin 5 antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722781) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Aquaporin 5 antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722781) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (negative) with Rabbit anti-Aquaporin 5 antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722781" style="font-weight: bold;text-decoration: underline;">HA722781</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (negative) with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722781) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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