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CRMP5 Recombinant Rabbit Monoclonal Antibody [JE34-69]

Usd: 385

Specification

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Catalog# HA722782

CRMP5 Recombinant Rabbit Monoclonal Antibody [JE34-69]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CRMP5 Recombinant Rabbit Monoclonal Antibody [JE34-69]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human CRMP5 aa 515-564 / 564.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 61 kDa

Positive Control

SH-SY5Y cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Rat brain tissue lysate, Rat testis tissue lysate.

Conjugation

unconjugated

Clone Number

JE34-69

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Dihydropyrimidinase-related protein 5 is an enzyme that in humans is encoded by the DPYSL5 gene. This gene encodes a member of the CRMP (collapsing response mediator protein) family thought to be involved in neural development. Antibodies to the encoded protein were found in some patients with neurologic symptoms who had paraneoplastic syndrome. A pseudogene of this gene is found on chromosome 11. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Background References

1. Totland C et al. CRMP5 Antibodies-Diagnostic Challenges. Front Neurol. 2021 Sep

2. Qiu F et al. CRMP5 regulates cell proliferation and development of colorectal cancer via MAPK-dependent signaling. Oncol Lett. 2021 Nov

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q9BPU6 Human

SwissProt: Q9EQF6 Mouse

SwissProt: Q9JHU0 Rat

Synonyms

Collapsin response mediator protein 5 antibody

CRAM antibody

CRMP 3 associated molecule antibody

CRMP 5 antibody

CRMP3 associated molecule antibody

Dihydropyrimidinase like 5 antibody

Dihydropyrimidinase related protein 5 antibody

Dihydropyrimidinase-related protein 5 antibody

DPYL5_HUMAN antibody

DPYS L5 antibody

Expand

Images

  • Western blot analysis of CRMP5 on different lysates with Rabbit anti-CRMP5 antibody (<a href="/products/HA722782" style="font-weight: bold;text-decoration: underline;">HA722782</a>) at 1/1,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 3: Mouse testis tissue lysate (20 µg/Lane)<br />Lane 4: Rat brain tissue lysate (20 µg/Lane)<br />Lane 5: Rat testis tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 61 kDa<br />Observed band size: 61 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722782" style="font-weight: bold;text-decoration: underline;">HA722782</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CRMP5 on different lysates with Rabbit anti-CRMP5 antibody (HA722782) at 1/1,000 dilution.

    Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 2: Mouse brain tissue lysate (20 µg/Lane)
    Lane 3: Mouse testis tissue lysate (20 µg/Lane)
    Lane 4: Rat brain tissue lysate (20 µg/Lane)
    Lane 5: Rat testis tissue lysate (20 µg/Lane)

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

    Exposure time: 30 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722782) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • CRMP5 was immunoprecipitated from 0.2 mg SH-SY5Y cell lysate with <a href="/products/HA722782" style="font-weight: bold;text-decoration: underline;">HA722782</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722782" style="font-weight: bold;text-decoration: underline;">HA722782</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: SH-SY5Y cell lysate (input)<br />Lane 2: <a href="/products/HA722782" style="font-weight: bold;text-decoration: underline;">HA722782</a> IP in SH-SY5Y cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722782" style="font-weight: bold;text-decoration: underline;">HA722782</a> in SH-SY5Y cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 2 seconds; ECL: <a href="/products/K1802" style="font-weight: bold;text-decoration: underline;">K1802</a>

    CRMP5 was immunoprecipitated from 0.2 mg SH-SY5Y cell lysate with HA722782 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722782 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: SH-SY5Y cell lysate (input)
    Lane 2: HA722782 IP in SH-SY5Y cell lysate
    Lane 3: Rabbit IgG instead of HA722782 in SH-SY5Y cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 2 seconds; ECL: K1802

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"