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Apolipoprotein CI / Apo-CI Recombinant Rabbit Monoclonal Antibody [JE36-30]

Usd: 385

Specification

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Catalog# HA722783

Apolipoprotein CI / Apo-CI Recombinant Rabbit Monoclonal Antibody [JE36-30]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Apolipoprotein CI / Apo-CI Recombinant Rabbit Monoclonal Antibody [JE36-30]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Apo-CI aa 1-83 / 83.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 9 kDa

Positive Control

HepG2 cell lysate, HepG2 treated with 300ng/mL BFA for 24 hours cell lysate.

Conjugation

unconjugated

Clone Number

JE36-30

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Apolipoprotein C-I is a protein component of lipoproteins that in humans is encoded by the APOC1 gene. The protein encoded by this gene is a member of the apolipoprotein C family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Background References

1. Hao X et al. Inhibition of APOC1 promotes the transformation of M2 into M1 macrophages via the ferroptosis pathway and enhances anti-PD1 immunotherapy in hepatocellular carcinoma based on single-cell RNA sequencing. Redox Biol. 2022 Oct

2. Ren L et al. Systematic pan-cancer analysis identifies APOC1 as an immunological biomarker which regulates macrophage polarization and promotes tumor metastasis. Pharmacol Res. 2022 Sep

Subcellular Location

Secreted.

UNIPROT

SwissProt: P02654 Human

Synonyms

APO C1 antibody

Apo CI antibody

Apo-CIB antibody

Apo-CIB' antibody

APOC 1 antibody

ApoC I antibody

ApoC-IB antibody

ApoC-IB' antibody

APOC1 antibody

APOC1_HUMAN antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Apolipoprotein CI / Apo-CI on different lysates with Rabbit anti-Apolipoprotein CI / Apo-CI antibody (<a href="/products/HA722783" style="font-weight: bold;text-decoration: underline;">HA722783</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: HepG2 treated with 300ng/mL BFA for 24 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 9 kDa<br />Observed band size: 9 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722783" style="font-weight: bold;text-decoration: underline;">HA722783</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Apolipoprotein CI / Apo-CI on different lysates with Rabbit anti-Apolipoprotein CI / Apo-CI antibody (HA722783) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: HepG2 treated with 300ng/mL BFA for 24 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 9 kDa
    Observed band size: 9 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722783) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"