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ERP27 Recombinant Rabbit Monoclonal Antibody [JE77-47]

Usd: 385

Specification

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Catalog# HA722784

ERP27 Recombinant Rabbit Monoclonal Antibody [JE77-47]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ERP27 Recombinant Rabbit Monoclonal Antibody [JE77-47]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ERP27 aa 1-200 / 273.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 30 kDa

Positive Control

Mouse pancreas tissue lysate, Rat pancreas tissue lysate, human pancreas tissue.

Conjugation

unconjugated

Clone Number

JE77-47

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:3,000

Target

Function

ERp27 (Endoplasmic Reticulum protein 27.7 kDa) is a homologue of PDI (protein disulfide-isomerase), localised to the Endoplasmic Reticulum. The structure of ERp27 has been solved by both X-ray crystallography and NMR spectroscopy, showing it to be composed of two thioredoxin-like domains with homology to the non-catalytic b and b' domains of PDI. The function of ERp27 is unknown, but on the basis of its homology with PDI it is thought to possess chaperone activity.

Background References

1. Amin NT et al. High-resolution NMR studies of structure and dynamics of human ERp27 indicate extensive interdomain flexibility. Biochem J. 2013 Mar

2. Alanen HI et al. ERp27, a new non-catalytic endoplasmic reticulum-located human protein disulfide isomerase family member, interacts with ERp57. J Biol Chem. 2006 Nov

Subcellular Location

Endoplasmic reticulum lumen.

UNIPROT

SwissProt: Q96DN0 Human

SwissProt: Q9D8U3 Mouse

Entrez Gene: 297698 Rat

Synonyms

C12orf46 antibody

Chromosome 12 open reading frame 46 antibody

Endoplasmic reticulum protein 27 antibody

Endoplasmic reticulum protein 27 kDa antibody

Endoplasmic reticulum resident protein 27 antibody

Endoplasmic reticulum resident protein ERp27 antibody

ER protein 27 antibody

ERp 27 antibody

ERp27 antibody

ERP27_HUMAN antibody

Expand

Images

  • Western blot analysis of ERP27 on different lysates with Rabbit anti-ERP27 antibody (<a href="/products/HA722784" style="font-weight: bold;text-decoration: underline;">HA722784</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse pancreas tissue lysate<br />Lane 2: Rat pancreas tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 30 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722784" style="font-weight: bold;text-decoration: underline;">HA722784</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ERP27 on different lysates with Rabbit anti-ERP27 antibody (HA722784) at 1/1,000 dilution.

    Lane 1: Mouse pancreas tissue lysate
    Lane 2: Rat pancreas tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 30 kDa
    Observed band size: 35 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722784) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-ERP27 antibody (<a href="/products/HA722784" style="font-weight: bold;text-decoration: underline;">HA722784</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722784" style="font-weight: bold;text-decoration: underline;">HA722784</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-ERP27 antibody (HA722784) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722784) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"