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Human IL-1RA Recombinant Rabbit Monoclonal Antibody [PSH07-04] - BSA and Azide free (Detector)

Usd: 649

Specification

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Catalog# HA722789

Human IL-1RA Recombinant Rabbit Monoclonal Antibody [PSH07-04] - BSA and Azide free (Detector)

Application

  • ELISA(Det)

Reactivity

  • Human

Pair: Capture
Pair: Detector
Pair: Protein
Range

  • 6.9-5,000 pg/mL

PI

  • 6.75

Conjugation

  • unconjugated

Overview

Product Name

Human IL-1RA Recombinant Rabbit Monoclonal Antibody [PSH07-04] - BSA and Azide free (Detector)

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human IL1RA aa 26-177 (HA210837).

Species Reactivity

Human

Validated Applications

ELISA(Det)

Target Molecular Weight

Predicted band size: 20 kDa

Positive Control

Recombinant human IL-1RA protein (HA210837).

Conjugation

unconjugated

Clone Number

PSH07-04

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • ELISA(Det)

  • Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-03] to Human IL-1RA antibody (Capture) (HA722788) and recombinant Human IL-1RA protein (HA210837) as the standard. The reference range value is 6.9-5,000 pg/mL.

Target

Function

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses, particularly in the acute phase of infection and inflammation. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.

Background References

1. Greenfeder S.A., Nunes P., Kwee L., Labow M., Chizzonite R.A., Ju G. Molecular cloning and characterization of a second subunit of the interleukin 1 receptor complex. J. Biol. Chem. 270:13757-13765 (1995)

2. Danis V.A., Millington M., Huang Q., Hyland V., Grennan D. Lack of association between an interleukin-1 receptor antagonist gene polymorphism and systemic lupus erythematosus. Dis Markers 12:135-139 (1995)

Subcellular Location

Secreted.

UNIPROT

SwissProt: P18510 Human

Synonyms

DIRA antibody

F630041P17Rik antibody

ICIL 1RA antibody

ICIL-1RA antibody

ICIL1RA antibody

IL-1ra antibody

IL-1ra3 antibody

IL-1RN antibody

IL1 inhibitor antibody

IL1F3 antibody

Expand

Images

  • Sandwich ELISA analysis of human IL-1RA matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA722788" style="font-weight: bold;text-decoration: underline;">HA722788</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant human IL-1RA protein (<a href="/products/HA210837" style="font-weight: bold;text-decoration: underline;">HA210837</a>) starting from 2000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA722789" style="font-weight: bold;text-decoration: underline;">HA722789</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Sandwich ELISA analysis of human IL-1RA matched pair antibodies
    
    Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722788) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant human IL-1RA protein (HA210837) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722789, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  • Interpolated concentrations of native IL-1RA in human samples.<br /><br />Interpolated concentration of native IL-1RA was measured in duplicate at different sample concentrations and interpolated from the IL-1RA standard curves. Undiluted samples were 50% samples. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1RA concentration was determined to be 4983 pg/mL in human urine.

    Interpolated concentrations of native IL-1RA in human samples.
    
    Interpolated concentration of native IL-1RA was measured in duplicate at different sample concentrations and interpolated from the IL-1RA standard curves. Undiluted samples were 50% samples. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1RA concentration was determined to be 4983 pg/mL in human urine.

  • Interpolated concentrations of native IL-1RA in human cell culture supernatant samples.<br /><br />Interpolated concentration of native IL-1RA was measured in duplicate at different sample concentrations and interpolated from the IL-1RA standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1RA concentration was determined to be 3068 pg/mL in THP-1 and 374 pg/ml in HepG2 in cell culture supernatant.

    Interpolated concentrations of native IL-1RA in human cell culture supernatant samples.
    
    Interpolated concentration of native IL-1RA was measured in duplicate at different sample concentrations and interpolated from the IL-1RA standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-1RA concentration was determined to be 3068 pg/mL in THP-1 and 374 pg/ml in HepG2 in cell culture supernatant.

  • Interpolated concentrations of spiked IL-1RA in human cell culture media samples.<br /><br />The concentrations of IL-1RA were measured in duplicates, interpolated from the IL-1RA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    Interpolated concentrations of spiked IL-1RA in human cell culture media samples.
    
    The concentrations of IL-1RA were measured in duplicates, interpolated from the IL-1RA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"