Phospho-Cytosolic Phospholipase A2 (S505) Recombinant Rabbit Monoclonal Antibody [PSH07-16]
Catalog# HA722804
Phospho-Cytosolic Phospholipase A2 (S505) Recombinant Rabbit Monoclonal Antibody [PSH07-16]
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WB
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Human
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Mouse
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HA751137
不含抗保成分
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unconjugated
Overview
Product Name
Phospho-Cytosolic Phospholipase A2 (S505) Recombinant Rabbit Monoclonal Antibody [PSH07-16]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser505 of human cPLA2 aa 491-540.
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 85 kDa
Positive Control
HeLa cell lysate, RAW264.7 cell lysates.
Conjugation
unconjugated
Clone Number
PSH07-16
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Cytosolic phospholipase A2 is an enzyme that in humans is encoded by the PLA2G4A gene. This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca2+ levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.
Background References
1. André S et al. Modulation of Cytosolic Phospholipase A2 as a Potential Therapeutic Strategy for Alzheimer\'s Disease. J Alzheimers Dis Rep. 2023 Dec
2. Zhao X et al. Cytosolic Phospholipase A2 Is Required for Fexofenadine\'s Therapeutic Effects against Inflammatory Bowel Disease in Mice. Int J Mol Sci. 2021 Oct
Subcellular Location
Cytoplasm, Golgi apparatus membrane, Nucleus envelope.
Synonyms
Calcium dependent phospholipid binding protein antibody
CPLA 2 antibody
cPLA2 alpha antibody
cPLA2 antibody
Cytosolic phospholipase A2 antibody
Cytosolic phospholipase A2 group IVA antibody
Lysophospholipase antibody
MGC126350 antibody
PA24A_HUMAN antibody
Phosphatidylcholine 2 acylhydrolase antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722804) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722804) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on RAW264.7 cell lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722804) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722804) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Phospho-Cytosolic Phospholipase A2 (S505) Recombinant Rabbit Monoclonal Antibody [PSH07-56] - BSA and Azide free
Application: WB,Dot Blot
Reactivity: Human,Mouse
Conjugate: unconjugated
Phospho-Cytosolic Phospholipase A2 (S505) Recombinant Rabbit Monoclonal Antibody [PSH07-16] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse
Conjugate: unconjugated
