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ANGPTL4 Recombinant Rabbit Monoclonal Antibody [PSH07-35]

Usd: 385

Specification

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Catalog# HA722823

ANGPTL4 Recombinant Rabbit Monoclonal Antibody [PSH07-35]

Application

  • WB

Reactivity

  • Human

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

ANGPTL4 Recombinant Rabbit Monoclonal Antibody [PSH07-35]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ANGPTL4 aa 107-406.

Species Reactivity

Human, Green monkey

Validated Applications

WB

Target Molecular Weight

Predicted band size: 45 kDa

Positive Control

A549 cell lysate, SW480 cell lysate, THP-1 cell lysate, HUVEC cell lysate, COS-1 cell lysate.

Conjugation

unconjugated

Clone Number

PSH07-35

Reactivity Data

WB
Human
Tested Tested
Green Monkey
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Angiopoietin-like 4 is a protein that in humans is encoded by the ANGPTL4 gene. Alternatively spliced transcript variants encoded with different isoforms have been described. This gene was previously referred to as ANGPTL2, HFARP, PGAR, or FIAF but has been renamed ANGPTL4. This gene is induced under hypoxic (low oxygen) condition in various cell types and is the target of peroxisome proliferator-activated receptors. The encoded protein is a serum hormone directly involved in regulating lipid metabolism. ANGPTL4 plays an important role in numerous cancers and is implicated in the metastatic process by modulating vascular permeability, cancer cell motility and invasiveness.

Background References

1. Zuo Y et al. Dual role of ANGPTL4 in inflammation. Inflamm Res. 2023 Jun

2. Singh AK et al. Hepatocyte-specific suppression of ANGPTL4 improves obesity-associated diabetes and mitigates atherosclerosis in mice. J Clin Invest. 2021 Jul

Subcellular Location

Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: Q9BY76 Human

Synonyms

Angiopoietin like 4 antibody

Angiopoietin related protein 4 antibody

Angiopoietin-like protein 4 antibody

Angiopoietin-related protein 4 antibody

ANGL4_HUMAN antibody

ANGPT L2 antibody

ANGPT L4 antibody

ANGPTL2 antibody

Angptl4 antibody

ARP4 antibody

Expand

Images

  • Western blot analysis of ANGPTL4 on different lysates with Rabbit anti-ANGPTL4 antibody (<a href="/products/HA722823" style="font-weight: bold;text-decoration: underline;">HA722823</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549 cell lysate<br />Lane 2: SW480 cell lysate<br />Lane 3: THP-1 cell lysate<br />Lane 4: HUVEC cell lysate<br />Lane 5: COS-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722823" style="font-weight: bold;text-decoration: underline;">HA722823</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ANGPTL4 on different lysates with Rabbit anti-ANGPTL4 antibody (HA722823) at 1/2,000 dilution.

    Lane 1: A549 cell lysate
    Lane 2: SW480 cell lysate
    Lane 3: THP-1 cell lysate
    Lane 4: HUVEC cell lysate
    Lane 5: COS-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 60 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722823) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ANGPTL4 on different lysates with Rabbit anti-ANGPTL4 antibody (<a href="/products/HA722823" style="font-weight: bold;text-decoration: underline;">HA722823</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-ANGPTL4 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722823" style="font-weight: bold;text-decoration: underline;">HA722823</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ANGPTL4 on different lysates with Rabbit anti-ANGPTL4 antibody (HA722823) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-ANGPTL4 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 60 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722823) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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