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MSH3 Recombinant Rabbit Monoclonal Antibody [PSH07-41]

Usd: 385

Specification

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Catalog# HA722829

MSH3 Recombinant Rabbit Monoclonal Antibody [PSH07-41]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

MSH3 Recombinant Rabbit Monoclonal Antibody [PSH07-41]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human MSH3 aa 201-600.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 127 kDa

Positive Control

HUVEC cell lysate, A549 cell lysate, HeLa cell lysate, NCI-H1299 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, SW480 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, F9 cell lysate, C6 cell lysate, L6 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, Rat colon tissue lysate, HeLa, human breast cancer tissue, human colon cancer tissue, human colon tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

PSH07-41

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:200

Target

Function

DNA mismatch repair protein, MutS Homolog 3 (MSH3) is a human homologue of the bacterial mismatch repair protein MutS that participates in the mismatch repair (MMR) system. MSH3 typically forms the heterodimer MutSβ with MSH2 in order to correct long insertion/deletion loops and base-base mispairs in microsatellites during DNA synthesis. Deficient capacity for MMR is found in approximately 15% of colorectal cancers, and somatic mutations in the MSH3 gene can be found in nearly 50% of MMR-deficient colorectal cancers.

Background References

1. O\'Reilly D et al. Di-valent siRNA-mediated silencing of MSH3 blocks somatic repeat expansion in mouse models of Huntington\'s disease. Mol Ther. 2023 Jun

2. Villy MC et al. MSH3: a confirmed predisposing gene for adenomatous polyposis. J Med Genet. 2023 Nov

Subcellular Location

Membrane, nucleoplasm, nucleus.

UNIPROT

SwissProt: P20585 Human

SwissProt: P13705 Mouse

Entrez Gene: 499505 Rat

Synonyms

Divergent upstream protein antibody

DNA mismatch repair protein antibody

DNA mismatch repair protein Msh 3 antibody

DNA mismatch repair protein MSH3 antibody

DUC 1 antibody

DUC1 antibody

DUG antibody

DUP antibody

hMSH3 antibody

MGC163306 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of MSH3 on different lysates with Rabbit anti-MSH3 antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: HUVEC cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: HeLa cell lysate (20 µg/Lane)<br />Lane 4: NCI-H1299 cell lysate (20 µg/Lane)<br />Lane 5: Jurkat cell lysate (20 µg/Lane)<br />Lane 6: HEK-293 cell lysate (20 µg/Lane)<br />Lane 7: HCT 116 cell lysate (negative) (20 µg/Lane)<br />Lane 8: SW480 cell lysate (20 µg/Lane)<br />Lane 9: HepG2 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 127 kDa<br />Observed band size: 127 kDa<br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of MSH3 on different lysates with Rabbit anti-MSH3 antibody (HA722829) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: HUVEC cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: HeLa cell lysate (20 µg/Lane)
    Lane 4: NCI-H1299 cell lysate (20 µg/Lane)
    Lane 5: Jurkat cell lysate (20 µg/Lane)
    Lane 6: HEK-293 cell lysate (20 µg/Lane)
    Lane 7: HCT 116 cell lysate (negative) (20 µg/Lane)
    Lane 8: SW480 cell lysate (20 µg/Lane)
    Lane 9: HepG2 cell lysate (20 µg/Lane)

    Predicted band size: 127 kDa
    Observed band size: 127 kDa
    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722829) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of MSH3 on different lysates with Rabbit anti-MSH3 antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 2: F9 cell lysate (20 µg/Lane)<br />Lane 3: C6 cell lysate (20 µg/Lane)<br />Lane 4: L6 cell lysate (20 µg/Lane)<br />Lane 5: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 6: Rat testis tissue lysate (40 µg/Lane)<br />Lane 7: Rat colon tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 127 kDa<br />Observed band size: 127 kDa<br />Exposure time: 59 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MSH3 on different lysates with Rabbit anti-MSH3 antibody (HA722829) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 2: F9 cell lysate (20 µg/Lane)
    Lane 3: C6 cell lysate (20 µg/Lane)
    Lane 4: L6 cell lysate (20 µg/Lane)
    Lane 5: Mouse testis tissue lysate (40 µg/Lane)
    Lane 6: Rat testis tissue lysate (40 µg/Lane)
    Lane 7: Rat colon tissue lysate (40 µg/Lane)

    Predicted band size: 127 kDa
    Observed band size: 127 kDa
    Exposure time: 59 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722829) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling MSH3 with Rabbit anti-MSH3 antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MSH3 antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling MSH3 with Rabbit anti-MSH3 antibody (HA722829) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MSH3 antibody (HA722829) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MSH3 antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MSH3 antibody (HA722829) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722829) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MSH3 antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MSH3 antibody (HA722829) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722829) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MSH3 antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MSH3 antibody (HA722829) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722829) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-MSH3 antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722829" style="font-weight: bold;text-decoration: underline;">HA722829</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-MSH3 antibody (HA722829) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722829) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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