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FOXP3 Recombinant Rabbit Monoclonal Antibody [PSH07-47]

Usd: 385

Specification

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Catalog# HA722835

FOXP3 Recombinant Rabbit Monoclonal Antibody [PSH07-47]

Application

  • WB

  • IHC-P

  • mIHC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

FOXP3 Recombinant Rabbit Monoclonal Antibody [PSH07-47]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human FOXP3 aa 101-370.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, mIHC

Molecular Weight

Predicted band size: 47 kDa

Positive Control

Mouse thymus tissue lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, human tonsil tissue, mouse spleen tissue, rat spleen tissue.

Conjugation

unconjugated

Clone Number

PSH07-47

Reactivity Data

WB IHC-P mIHC
Human
Not Recommended
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

  • mIHC

  • 1:100-1:800

Target

Function

FOXP3 (forkhead box P3), also known as scurfin, is a protein involved in immune system responses. A member of the FOX protein family, FOXP3 appears to function as a master regulator of the regulatory pathway in the development and function of regulatory T cells. Regulatory T cells generally turn the immune response down. In cancer, an excess of regulatory T cell activity can prevent the immune system from destroying cancer cells. In autoimmune disease, a deficiency of regulatory T cell activity can allow other autoimmune cells to attack the body's own tissues.

Background References

1. Wang J et al. Human FOXP3 and tumour microenvironment. Immunology. 2023 Feb

2. Ziółkowska-Suchanek I et al. FOXP3: A Player of Immunogenetic Architecture in Lung Cancer. Genes (Basel). 2024 Apr

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q9BZS1 Human

SwissProt: Q99JB6 Mouse

Entrez Gene: 317382 Rat

Synonyms

AIID antibody

DIETER antibody

Forkhead box P3 antibody

Forkhead box protein P3 antibody

FOXP3 antibody

FOXP3_HUMAN antibody

FOXP3delta7 antibody

Immune dysregulation polyendocrinopathy enteropathy X linked antibody

Immunodeficiency polyendocrinopathy enteropathy X linked antibody

IPEX antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of FOXP3 on different lysates with Rabbit anti-FOXP3 antibody (<a href="/products/HA722835" style="font-weight: bold;text-decoration: underline;">HA722835</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse thymus tissue lysate<br />Lane 2: Mouse spleen tissue lysate<br />Lane 3: Mouse liver tissue lysate (negative)<br />Lane 4: Rat spleen tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 47 kDa<br />Observed band size: 47 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722835" style="font-weight: bold;text-decoration: underline;">HA722835</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of FOXP3 on different lysates with Rabbit anti-FOXP3 antibody (HA722835) at 1/1,000 dilution.

    Lane 1: Mouse thymus tissue lysate
    Lane 2: Mouse spleen tissue lysate
    Lane 3: Mouse liver tissue lysate (negative)
    Lane 4: Rat spleen tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722835) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FOXP3 antibody (<a href="/products/HA722835" style="font-weight: bold;text-decoration: underline;">HA722835</a>) at 1/500 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FOXP3 antibody (HA722835) at 1/500 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-FOXP3 antibody (<a href="/products/HA722835" style="font-weight: bold;text-decoration: underline;">HA722835</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722835" style="font-weight: bold;text-decoration: underline;">HA722835</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-FOXP3 antibody (HA722835) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722835) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-FOXP3 antibody (<a href="/products/HA722835" style="font-weight: bold;text-decoration: underline;">HA722835</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722835" style="font-weight: bold;text-decoration: underline;">HA722835</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-FOXP3 antibody (HA722835) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722835) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-FOXP3 antibody (<a href="/products/HA722835" style="font-weight: bold;text-decoration: underline;">HA722835</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722835" style="font-weight: bold;text-decoration: underline;">HA722835</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-FOXP3 antibody (HA722835) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722835) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-FOXP3 antibody (<a href="/products/HA722835" style="font-weight: bold;text-decoration: underline;">HA722835</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722835" style="font-weight: bold;text-decoration: underline;">HA722835</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-FOXP3 antibody (HA722835) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722835) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation