NrCAM Recombinant Rabbit Monoclonal Antibody [JE30-78]
Catalog# HA722843
NrCAM Recombinant Rabbit Monoclonal Antibody [JE30-78]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NrCAM Recombinant Rabbit Monoclonal Antibody [JE30-78]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human NrCAM aa 821-970 / 1,304.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 144 kDa
Positive Control
SH-SY5Y cell lysate, A549 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue lysates.
Conjugation
unconjugated
Clone Number
JE30-78
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.
Background References
1. Bai C, Chen DG. NRCAM acts as a prognostic biomarker and promotes the tumor progression in gastric cancer via EMT pathway. Tissue Cell. 2022 Aug;77:101859. doi: 10.1016/j.tice.2022.101859. Epub 2022 Jun 22. PMID: 35763884.
2. Brummer T, Müller SA, Pan-Montojo F, Yoshida F, Fellgiebel A, Tomita T, Endres K, Lichtenthaler SF. NrCAM is a marker for substrate-selective activation of ADAM10 in Alzheimer\'s disease. EMBO Mol Med. 2019 Apr;11(4):e9695. doi: 10.15252/emmm.201809695. PMID: 30833305; PMCID: PMC6460357.
Subcellular Location
Cell membrane,Cell projection,,Secreted
Synonyms
Bravo antibody
hBravo antibody
Neuronal cell adhesion molecule antibody
Neuronal surface protein Bravo antibody
Ng CAM related antibody
Ng-CAM-related antibody
NgCAM related cell adhesion molecule antibody
NgCAM-related cell adhesion molecule antibody
Nr CAM antibody
Nr-CAM antibody
ExpandImages
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Western blot analysis of NrCAM on different lysates with Rabbit anti-NrCAM antibody (HA722843) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (30 µg/Lane)
Lane 4: Rat brain tissue lysate (30 µg/Lane)
Predicted band size: 144 kDa
Observed band size: 144 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722843) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of NrCAM on human brain tissue lysates with Rabbit anti-NrCAM antibody (HA722843) at 1/1,000 dilution.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 144 kDa
Observed band size: 144 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722843) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"