DDIT3 Recombinant Rabbit Monoclonal Antibody [PSH07-65] (HA722854)
Catalog# HA722854
Rabbit Monoclonal to DDIT3
Tested for
-
WB
-
IF-Cell
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FC
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (HA722854) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 2μg/mL tunicamycin for 8 hours cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C2C12 treated with 2μg/mL thapsigargin for 8 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 2μg/mL tunicamycin for 8 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 27 kDa
Exposure time: Lane 1-2: 3 minutes; Lane 3-6: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722854) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 2μg/mL tunicamycin for 8 hours cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C2C12 treated with 2μg/mL thapsigargin for 8 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 2μg/mL tunicamycin for 8 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 27 kDa
Exposure time: Lane 1-2: 3 minutes; Lane 3-6: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722854) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.