KLRG1 Recombinant Rabbit Monoclonal Antibody [JE33-71]
Catalog# HA722859
KLRG1 Recombinant Rabbit Monoclonal Antibody [JE33-71]
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WB
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Human
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Rat
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Mouse
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unconjugated
Overview
Product Name
KLRG1 Recombinant Rabbit Monoclonal Antibody [JE33-71]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human KLRG1 aa 1-38 / 195.
Species Reactivity
Human, Rat, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 22 kDa
Positive Control
NK-92 cell lysate, Jurkat cell lysate, MoLT-4 cell lysate, EL4 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate.
Conjugation
unconjugated
Clone Number
JE33-71
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Rat |
|
| Mouse |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Plays an inhibitory role on natural killer (NK) cells and T-cell functions upon binding to their non-MHC ligands. May mediate missing self recognition by binding to a highly conserved site on classical cadherins, enabling it to monitor expression of E-cadherin/CDH1, N-cadherin/CDH2 and R-cadherin/CDH4 on target cells.
Background References
1. Herndler-Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, Lietzenmayer M, Kroehling L, Takumi A, Kometani K, Inoue T, Kluger Y, Kaech SM, Kurosaki T, Okada T, Flavell RA. KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity. Immunity. 2018 Apr 17;48(4):716-729.e8. doi: 10.1016/j.immuni.2018.03.015. Epub 2018 Apr 3. PMID: 29625895; PMCID: PMC6465538.
2. Ager CR, Zhang M, Chaimowitz M, Bansal S, Tagore S, Obradovic A, Jugler C, Rogava M, Melms JC, McCann P, Spina C, Drake CG, Dallos MC, Izar B. KLRG1 marks tumor-infiltrating CD4 T cell subsets associated with tumor progression and immunotherapy response. J Immunother Cancer. 2023 Sep;11(9):e006782. doi: 10.1136/jitc-2023-006782. PMID: 37657842; PMCID: PMC10476134.
Subcellular Location
Cell membrane.
Synonyms
2F1 Ag antibody
2F1 antibody
C type lectin domain family 15 member A antibody
C-type lectin domain family 15 member A antibody
CLEC15A antibody
ITIM containing receptor MAFA L antibody
ITIM-containing receptor MAFA-L antibody
Killer cell lectin like receptor G1 antibody
Killer cell lectin like receptor subfamily G member 1 antibody
Killer cell lectin-like receptor subfamily G member 1 antibody
ExpandImages
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Western blot analysis of KLRG1 on different lysates with Rabbit anti-KLRG1 antibody (HA722859) at 1/1,000 dilution.
Lane 1: NK-92 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MoLT-4 cell lysate
Lane 4: EL4 cell lysate
Lane 5: mouse spleen tissue lysate
Lane 6: rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 35 kDa,75 kDa
Exposure time: 1 minutes 34 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722859) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"