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TMEFF2 Recombinant Rabbit Monoclonal Antibody [JE39-42]

Usd: 385

Specification

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Catalog# HA722861

TMEFF2 Recombinant Rabbit Monoclonal Antibody [JE39-42]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

TMEFF2 Recombinant Rabbit Monoclonal Antibody [JE39-42]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human TMEFF2 aa 342-374 / 374.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 41 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, LNCaP cell lysate, 22RV1 cell lysate, RWPE-1 cell lysate, human colon tissue, human prostate tissue.

Conjugation

unconjugated

Clone Number

JE39-42

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.

Background References

1. Xie S, Zhang Y, Peng T, Guo J, Cao Y, Guo J, Shi X, Li Y, Liu Y, Qi S, Wang H. TMEFF2 promoter hypermethylation is an unfavorable prognostic marker in gliomas. Cancer Cell Int. 2021 Mar 4;21(1):148. doi: 10.1186/s12935-021-01818-x. PMID: 33663520; PMCID: PMC7931334.

2. Sun T, Du W, Xiong H, Yu Y, Weng Y, Ren L, Zhao H, Wang Y, Chen Y, Xu J, Xiang Y, Qin W, Cao W, Zou W, Chen H, Hong J, Fang JY. TMEFF2 deregulation contributes to gastric carcinogenesis and indicates poor survival outcome. Clin Cancer Res. 2014 Sep 1;20(17):4689-704. doi: 10.1158/1078-0432.CCR-14-0315. Epub 2014 Jul 1. Erratum in: Clin Cancer Res. 2015 Aug 1;21(15):3572. doi: 10.1158/1078-0432.CCR-15-1270. PMID: 24987055.

Subcellular Location

Membrane, Secreted.

UNIPROT

SwissProt: Q9UIK5 Human

Synonyms

HPP1 antibody

TENB2 antibody

TMEFF2 antibody

Tomoregulin 2 antibody

TPEF antibody

TR antibody

TR 2 antibody

Images

  • Western blot analysis of TMEFF2 on different lysates with Rabbit anti-TMEFF2 antibody (<a href="/products/HA722861" style="font-weight: bold;text-decoration: underline;">HA722861</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: LNCaP cell lysate (20 µg/Lane)<br />Lane 4: 22RV1 cell lysate (20 µg/Lane)<br />Lane 5: RWPE-1 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41/45 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722861" style="font-weight: bold;text-decoration: underline;">HA722861</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TMEFF2 on different lysates with Rabbit anti-TMEFF2 antibody (HA722861) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: LNCaP cell lysate (20 µg/Lane)
    Lane 4: 22RV1 cell lysate (20 µg/Lane)
    Lane 5: RWPE-1 cell lysate (20 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 41/45 kDa

    Exposure time: 42 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722861) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TMEFF2 antibody (<a href="/products/HA722861" style="font-weight: bold;text-decoration: underline;">HA722861</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722861" style="font-weight: bold;text-decoration: underline;">HA722861</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TMEFF2 antibody (HA722861) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722861) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-TMEFF2 antibody (<a href="/products/HA722861" style="font-weight: bold;text-decoration: underline;">HA722861</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722861" style="font-weight: bold;text-decoration: underline;">HA722861</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-TMEFF2 antibody (HA722861) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722861) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"