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FSH beta Recombinant Rabbit Monoclonal Antibody [JE60-54]

Usd: 385

Specification

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Catalog# HA722866

FSH beta Recombinant Rabbit Monoclonal Antibody [JE60-54]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

FSH beta Recombinant Rabbit Monoclonal Antibody [JE60-54]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human FSH beta aa 41-90 / 129.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 15 kDa

Positive Control

Mouse pituitary tissue lysate, Rat pituitary tissue lysate, Rat heart tissue lysate, human pituitary tumor tissue, rat pituitary tissue, mouse pituitary tissue.

Conjugation

unconjugated

Clone Number

JE60-54

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Follitropin subunit beta also known as follicle-stimulating hormone beta subunit (FSH-B) is a protein that in humans is encoded by the FSHB gene. Alternative splicing results in two transcript variants encoding the same protein. The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production.

Background References

1. Bohaczuk SC et al. FSHB Transcription is Regulated by a Novel 5\' Distal Enhancer With a Fertility-Associated Single Nucleotide Polymorphism. Endocrinology. 2021 Jan

2. Krenz H et al. FSHB Genotype Identified as a Relevant Diagnostic Parameter Revealed by Cluster Analysis of Men With Idiopathic Infertility. Front Endocrinol (Lausanne). 2021 Dec

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01225 Human

SwissProt: Q60687 Mouse

SwissProt: P18427 Rat

Synonyms

Follicle Stimulating Hormone antibody

Follicle stimulating hormone beta polypeptide antibody

Follicle stimulating hormone beta subunit antibody

Follitropin beta chain antibody

Follitropin subunit beta antibody

FSH-B antibody

FSH-beta antibody

FSHB antibody

FSHbeta antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of FSH beta on different lysates with Rabbit anti-FSH beta antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse pituitary tissue lysate<br />Lane 2: Mouse spleen tissue lysate (negative)<br />Lane 3: Mouse heart tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 15 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of FSH beta on different lysates with Rabbit anti-FSH beta antibody (HA722866) at 1/1,000 dilution.

    Lane 1: Mouse pituitary tissue lysate
    Lane 2: Mouse spleen tissue lysate (negative)
    Lane 3: Mouse heart tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 15 kDa
    Observed band size: 25 kDa

    Exposure time: 1 minute; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722866) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of FSH beta on different lysates with Rabbit anti-FSH beta antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/1,000 dilution.<br /><br />Lane 1: Rat pituitary tissue lysate<br />Lane 2: Rat spleen tissue lysate (negative)<br />Lane 3: Rat heart tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 15 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 59 seconds; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of FSH beta on different lysates with Rabbit anti-FSH beta antibody (HA722866) at 1/1,000 dilution.

    Lane 1: Rat pituitary tissue lysate
    Lane 2: Rat spleen tissue lysate (negative)
    Lane 3: Rat heart tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 15 kDa
    Observed band size: 25 kDa

    Exposure time: 59 seconds; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722866) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human pituitary tumor tissue with Rabbit anti-FSH beta antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pituitary tumor tissue with Rabbit anti-FSH beta antibody (HA722866) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722866) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-FSH beta antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-FSH beta antibody (HA722866) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722866) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-FSH beta antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-FSH beta antibody (HA722866) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722866) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-FSH beta antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722866" style="font-weight: bold;text-decoration: underline;">HA722866</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-FSH beta antibody (HA722866) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722866) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

FSH beta Rabbit Polyclonal Antibody

Application: WB,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated