LCAT Recombinant Rabbit Monoclonal Antibody [JE35-16]
Catalog# HA722868
LCAT Recombinant Rabbit Monoclonal Antibody [JE35-16]
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WB
-
IHC-P
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Mouse
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Rat
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unconjugated
Overview
Product Name
LCAT Recombinant Rabbit Monoclonal Antibody [JE35-16]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within mouse LCAT aa 1-50 / 438.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 50 kDa
Positive Control
Mouse liver tissue lysate, Rat liver tissue lysate, rat liver tissue.
Conjugation
unconjugated
Clone Number
JE35-16
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
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IHC-P
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1:1,000
Target
Function
Lecithin–cholesterol acyltransferase (LCAT, also called phosphatidylcholine–sterol O-acyltransferase) is an enzyme, in many animals including humans, that converts free cholesterol into cholesteryl ester (a more hydrophobic form of cholesterol), which is then sequestered into the core of a lipoprotein particle, eventually making the newly synthesized HDL spherical and forcing the reaction to become unidirectional since the particles are removed from the surface. The enzyme is bound to high-density lipoproteins (HDLs) (alpha-LCAT) and LDLs (beta-LCAT) in the blood plasma. LCAT deficiency can cause impaired vision due to cholesterol corneal opacities, anemia, and kidney damage. It belongs to the family of phospholipid:diacylglycerol acyltransferases.
Background References
1. Yang K et al. LCAT- targeted therapies: Progress, failures and future. Biomed Pharmacother. 2022 Mar
2. Lin X et al. Depleting LCAT Aggravates Atherosclerosis in LDLR-deficient Hamster with Reduced LDL-Cholesterol Level. J Adv Res. 2023 Nov
Subcellular Location
Secreted.
Synonyms
LCAT antibody
LCAT_HUMAN antibody
Lecithin cholesterol acyltransferase antibody
Lecithin-cholesterol acyltransferase antibody
Phosphatidylcholine sterol acyltransferase antibody
Phosphatidylcholine-sterol acyltransferase antibody
Phospholipid cholesterol acyltransferase antibody
Phospholipid-cholesterol acyltransferase antibody
Images
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Western blot analysis of LCAT on different lysates with Rabbit anti-LCAT antibody (HA722868) at 1/1,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722868) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-LCAT antibody (HA722868) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722868) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"