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Connexin 32 / GJB1 Recombinant Rabbit Monoclonal Antibody [JE54-70]

Usd: 385

Specification

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Catalog# HA722869

Connexin 32 / GJB1 Recombinant Rabbit Monoclonal Antibody [JE54-70]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Connexin 32 / GJB1 Recombinant Rabbit Monoclonal Antibody [JE54-70]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Connexin 32 / GJB1 aa 46-75 / 283.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 32 kDa

Positive Control

MCF7 cell lysate, 293T cell lysate, Human lung tissue lysate, Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

JE54-70

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P IF-Cell
human
Tested Tested
Not Recommended
Not Recommended
mouse
Tested Tested
Not Recommended
 
rat
Tested Tested
Not Recommended
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

Gap junction beta-1 protein (GJB1), also known as connexin 32 (Cx32), is a transmembrane protein that in humans is encoded by the GJB1 gene. Gap junction beta-1 protein is a member of the gap junction connexin family of proteins that regulates and controls the transfer of communication signals across cell membranes, primarily in the liver and peripheral nervous system. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. For a general discussion of connexin proteins, see GJB2. In Schwann cells, GJB1 also forms channels that facilitate transfers between layers of the myelin. In melanocytic cells GJB1 gene expression may be regulated by MITF.

Background References

1. Wu S et al. Connexin 32 deficiency protects the liver against ischemia/reperfusion injury. Eur J Pharmacol. 2020 Jun

2. Ugur D et al. Connexin 32 overexpression increases proliferation, reduces gap junctional intercellular communication, motility and epithelial-to-mesenchymal transition in Hs578T breast cancer cells. J Cell Commun Signal. 2022 Sep

Subcellular Location

Cell membrane, Cell junction, gap junction.

UNIPROT

SwissProt: P08034 Human

SwissProt: P28230 Mouse

SwissProt: P08033 Rat

Synonyms

Charcot Marie Tooth neuropathy X linked antibody

CMTX 1 antibody

CMTX antibody

CMTX1 antibody

Connexin-32 antibody

Connexin32 antibody

CX 32 antibody

Cx32 antibody

CXB1_HUMAN antibody

GAP junction 28 kDa liver protein antibody

Expand

Images

  • Western blot analysis of Connexin 32 / GJB1 on different lysates with Rabbit anti-Connexin 32 / GJB1 antibody (<a href="/products/HA722869" style="font-weight: bold;text-decoration: underline;">HA722869</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate (20 µg/Lane)<br />Lane 2: 293T cell lysate (20 µg/Lane)<br />Lane 3: Human lung tissue lysate (40 µg/Lane)<br />Lane 4: Human liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 32 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722869" style="font-weight: bold;text-decoration: underline;">HA722869</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Connexin 32 / GJB1 on different lysates with Rabbit anti-Connexin 32 / GJB1 antibody (HA722869) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate (20 µg/Lane)
    Lane 2: 293T cell lysate (20 µg/Lane)
    Lane 3: Human lung tissue lysate (40 µg/Lane)
    Lane 4: Human liver tissue lysate (40 µg/Lane)

    Predicted band size: 32 kDa
    Observed band size: 26 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722869) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Connexin 32 / GJB1 on different lysates with Rabbit anti-Connexin 32 / GJB1 antibody (<a href="/products/HA722869" style="font-weight: bold;text-decoration: underline;">HA722869</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse liver tissue lysate<br />Lane 2: Mouse spleen tissue lysate (negative)<br />Lane 3: Rat liver tissue lysate<br />Lane 4: Rat spleen tissue lysate (negative)<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 32 kDa<br />Observed band size: 48 kDa<br /><br />Exposure time: 1 minute 46 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722869" style="font-weight: bold;text-decoration: underline;">HA722869</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Connexin 32 / GJB1 on different lysates with Rabbit anti-Connexin 32 / GJB1 antibody (HA722869) at 1/2,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Mouse spleen tissue lysate (negative)
    Lane 3: Rat liver tissue lysate
    Lane 4: Rat spleen tissue lysate (negative)

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 32 kDa
    Observed band size: 48 kDa

    Exposure time: 1 minute 46 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722869) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"