Human Pro-MMP-1 Recombinant Rabbit Monoclonal Antibody [PSH07-59] - BSA and Azide free (Capture)
Overview
Product Name
Human Pro-MMP-1 Recombinant Rabbit Monoclonal Antibody [PSH07-59] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Pro-MMP-1 aa 20-469 (HA210903).
Species Reactivity
Human
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Human Pro-MMP-1 protein (HA210903).
Conjugation
unconjugated
Clone Number
PSH07-59
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-60] to Human Pro-MMP-1 (Detecor) (HA722875) and Recombinant Human Pro-MMP-1 protein (HA210903) as the standard. The reference range value is 37-3,000 pg/mL.
Target
Function
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Background References
1. Rumbaugh J., Turchan-Cholewo J., Galey D., St Hillaire C., Anderson C., Conant K., Nath A. Interaction of HIV Tat and matrix metalloproteinase in HIV neuropathogenesis: a new host defense mechanism. FASEB J. 20:1736-1738 (2006)
2. Clark I.M., Cawston T.E. Fragments of human fibroblast collagenase. Purification and characterization. Biochem. J. 263:201-206 (1989)
Subcellular Location
Secreted.
UNIPROT
Synonyms
27 kDa interstitial collagenase antibody
CLG antibody
CLGN antibody
collagenase, fibroblast antibody
collagenase, interstitial antibody
Fibroblast collagenase antibody
Interstitial collagenase antibody
Matrix metallopeptidase 1 (interstitial collagenase) antibody
Matrix metalloprotease 1 antibody
Matrix Metalloproteinase 1 antibody
ExpandImages
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![Sandwich ELISA analysis of Human Pro-MMP-1 matched pair antibodies<br /><br />Capture: <a href="/products/HA722874" style="font-weight: bold;text-decoration: underline;">HA722874</a>, Human Pro-MMP-1 Rabbit mAb [PSH07-59]<br />Detector: <a href="/products/HA722875" style="font-weight: bold;text-decoration: underline;">HA722875</a>, Human Pro-MMP-1 Rabbit mAb [PSH07-60]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA722874" style="font-weight: bold;text-decoration: underline;">HA722874</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Pro-MMP-1 protein (<a href="/products/HA210903" style="font-weight: bold;text-decoration: underline;">HA210903</a>) starting from 3,000 pg/mL to 0 pg/mL and detect antibody (<a href="/products/HA722875" style="font-weight: bold;text-decoration: underline;">HA722875</a>, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA722874_1.png?v=20260908003247)
Sandwich ELISA analysis of Human Pro-MMP-1 matched pair antibodies
Capture: HA722874, Human Pro-MMP-1 Rabbit mAb [PSH07-59]
Detector: HA722875, Human Pro-MMP-1 Rabbit mAb [PSH07-60]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722874) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Pro-MMP-1 protein (HA210903) starting from 3,000 pg/mL to 0 pg/mL and detect antibody (HA722875, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Pro-MMP-1 in human cell culture supernatant samples.<br /><br />Capture: <a href="/products/HA722874" style="font-weight: bold;text-decoration: underline;">HA722874</a>, Human Pro-MMP-1 Rabbit mAb [PSH07-59]<br />Detector: <a href="/products/HA722875" style="font-weight: bold;text-decoration: underline;">HA722875</a>, Human Pro-MMP-1 Rabbit mAb [PSH07-60]<br /><br />Interpolated concentration of native Pro-MMP-1 was measured in duplicate at different sample concentrations and interpolated from the Pro-MMP-1 standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Pro-MMP-1 concentration was determined to be 2,316 pg/mL in SW480 and 1,660 pg/mL in HepG2 in cell culture supernatant, undetectable in A549 supernatant.](https://storage.huabio.cn/huabio/productImg/HA722874_2.png?v=20260908003247)
Interpolated concentrations of native Pro-MMP-1 in human cell culture supernatant samples.
Capture: HA722874, Human Pro-MMP-1 Rabbit mAb [PSH07-59]
Detector: HA722875, Human Pro-MMP-1 Rabbit mAb [PSH07-60]
Interpolated concentration of native Pro-MMP-1 was measured in duplicate at different sample concentrations and interpolated from the Pro-MMP-1 standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Pro-MMP-1 concentration was determined to be 2,316 pg/mL in SW480 and 1,660 pg/mL in HepG2 in cell culture supernatant, undetectable in A549 supernatant. -
![Interpolated concentrations of spiked Pro-MMP-1 in human cell culture media samples.<br /><br />Capture: <a href="/products/HA722874" style="font-weight: bold;text-decoration: underline;">HA722874</a>, Human Pro-MMP-1 Rabbit mAb [PSH07-59]<br />Detector: <a href="/products/HA722875" style="font-weight: bold;text-decoration: underline;">HA722875</a>, Human Pro-MMP-1 Rabbit mAb [PSH07-60]<br /><br />The concentrations of Pro-MMP-1 were measured in duplicates, interpolated from the Pro-MMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA722874_3.png?v=20260908003247)
Interpolated concentrations of spiked Pro-MMP-1 in human cell culture media samples.
Capture: HA722874, Human Pro-MMP-1 Rabbit mAb [PSH07-59]
Detector: HA722875, Human Pro-MMP-1 Rabbit mAb [PSH07-60]
The concentrations of Pro-MMP-1 were measured in duplicates, interpolated from the Pro-MMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"