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TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH07-75]

Usd: 385

Specification

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Catalog# HA722891

TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH07-75]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH07-75]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TIM 3 aa 1-223.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 33 kDa

Positive Control

RPMI 8226 cell lysate, HUT 102 cell lysate.

Conjugation

unconjugated

Clone Number

PSH07-75

Reactivity Data

WB
Human
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:4,000

Target

Function

Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 (TIM-3)gene. HAVCR2 was first described in 2002 as a cell surface molecule expressed on IFNγ producing CD4+ Th1 and CD8+ Tc1 cells. Later, the expression was detected in Th17 cells, regulatory T-cells, and innate immune cells (dendritic cells, NK cells, monocytes, macrophages). HAVCR2 receptor is a regulator of the immune response.

Background References

1. Kandel S et al. The TIM3/Gal9 signaling pathway: An emerging target for cancer immunotherapy. Cancer Lett. 2021 Jul

2. Palacios LM et al. TIM3 Expression in Anaplastic-Thyroid-Cancer-Infiltrating Macrophages: An Emerging Immunotherapeutic Target. Biology (Basel). 2022 Nov

Subcellular Location

Membrane, Cell junction, Cell membrane.

UNIPROT

SwissProt: Q8TDQ0 Human

Synonyms

CD366 antibody

FLJ14428 antibody

HAVcr-2 antibody

Havcr2 antibody

HAVR2_HUMAN antibody

Hepatitis A virus cellular receptor 2 antibody

Kidney injury molecule 3 antibody

KIM 3 antibody

KIM3 antibody

T cell immunoglobulin and mucin domain containing 3 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TIM 3 on different lysates with Rabbit anti-TIM 3 antibody (<a href="/products/HA722891" style="font-weight: bold;text-decoration: underline;">HA722891</a>) at 1/4,000 dilution.<br /><br />Lane 1: RPMI 8226 cell lysate (20 µg/Lane)<br />Lane 2: HUT 102 cell lysate (40 µg/Lane)<br />Lane 3: HT-29 cell lysate (negative) (40 µg/Lane)<br /><br />Predicted band size: 33 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722891" style="font-weight: bold;text-decoration: underline;">HA722891</a>) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TIM 3 on different lysates with Rabbit anti-TIM 3 antibody (HA722891) at 1/4,000 dilution.

    Lane 1: RPMI 8226 cell lysate (20 µg/Lane)
    Lane 2: HUT 102 cell lysate (40 µg/Lane)
    Lane 3: HT-29 cell lysate (negative) (40 µg/Lane)

    Predicted band size: 33 kDa
    Observed band size: 55 kDa

    Exposure time: 42 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722891) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"