TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH07-75]
Catalog# HA722891
TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH07-75]
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WB
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Human
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unconjugated
Overview
Product Name
TIM 3 Recombinant Rabbit Monoclonal Antibody [PSH07-75]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TIM 3 aa 1-223.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 33 kDa
Positive Control
RPMI 8226 cell lysate, HUT 102 cell lysate.
Conjugation
unconjugated
Clone Number
PSH07-75
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:4,000
Target
Function
Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 (TIM-3)gene. HAVCR2 was first described in 2002 as a cell surface molecule expressed on IFNγ producing CD4+ Th1 and CD8+ Tc1 cells. Later, the expression was detected in Th17 cells, regulatory T-cells, and innate immune cells (dendritic cells, NK cells, monocytes, macrophages). HAVCR2 receptor is a regulator of the immune response.
Background References
1. Kandel S et al. The TIM3/Gal9 signaling pathway: An emerging target for cancer immunotherapy. Cancer Lett. 2021 Jul
2. Palacios LM et al. TIM3 Expression in Anaplastic-Thyroid-Cancer-Infiltrating Macrophages: An Emerging Immunotherapeutic Target. Biology (Basel). 2022 Nov
Subcellular Location
Membrane, Cell junction, Cell membrane.
UNIPROT
Synonyms
CD366 antibody
FLJ14428 antibody
HAVcr-2 antibody
Havcr2 antibody
HAVR2_HUMAN antibody
Hepatitis A virus cellular receptor 2 antibody
Kidney injury molecule 3 antibody
KIM 3 antibody
KIM3 antibody
T cell immunoglobulin and mucin domain containing 3 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of TIM 3 on different lysates with Rabbit anti-TIM 3 antibody (HA722891) at 1/4,000 dilution.
Lane 1: RPMI 8226 cell lysate (20 µg/Lane)
Lane 2: HUT 102 cell lysate (40 µg/Lane)
Lane 3: HT-29 cell lysate (negative) (40 µg/Lane)
Predicted band size: 33 kDa
Observed band size: 55 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722891) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"