CD79a Recombinant Rabbit Monoclonal Antibody [JE32-14]
Catalog# HA722896
CD79a Recombinant Rabbit Monoclonal Antibody [JE32-14]
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WB
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IF-Cell
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
CD79a Recombinant Rabbit Monoclonal Antibody [JE32-14]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CD79a aa 51-100 / 226.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 25 kDa
Positive Control
Ramos cell lysate, Daudi cell lysate, Raji cell lysate, Daudi, human colon tissue, human lung cancer tissue, human spleenr tissue.
Conjugation
unconjugated
Clone Number
JE32-14
Reactivity Data
| WB | IF-Cell | IHC-P | IF-Tissue | |
|---|---|---|---|---|
| Human |
|
|
|
|
| Mouse |
|
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| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
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IF-Cell
-
1:50
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IHC-P
-
1:200-1:1,000
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IF-Tissue
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1:50
Target
Function
Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.
Background References
1. Lenk L, Carlet M, Vogiatzi F, Spory L, Winterberg D, Cousins A, Vossen-Gajcy M, Ibruli O, Vokuhl C, Cario G, El Ayoubi O, Kramer L, Ritgen M, Brüggemann M, Häsler R, Schrappe M, Fuhrmann S, Halsey C, Jeremias I, Hobeika E, Jumaa H, Alsadeq A, Schewe DM. CD79a promotes CNS-infiltration and leukemia engraftment in pediatric B-cell precursor acute lymphoblastic leukemia. Commun Biol. 2021 Jan 15;4(1):73. doi: 10.1038/s42003-020-01591-z. PMID: 33452446; PMCID: PMC7810877.
2. Lenk L, Carlet M, Vogiatzi F, Spory L, Winterberg D, Cousins A, Vossen-Gajcy M, Ibruli O, Vokuhl C, Cario G, El Ayoubi O, Kramer L, Ritgen M, Brüggemann M, Häsler R, Schrappe M, Fuhrmann S, Halsey C, Jeremias I, Hobeika E, Jumaa H, Alsadeq A, Schewe DM. CD79a promotes CNS-infiltration and leukemia engraftment in pediatric B-cell precursor acute lymphoblastic leukemia. Commun Biol. 2021 Jan 15;4(1):73. doi: 10.1038/s42003-020-01591-z. PMID: 33452446; PMCID: PMC7810877.
Subcellular Location
Cell membrane
UNIPROT
Synonyms
B lymphocyte-specific MB1 protein antibody
B-cell antigen receptor complex-associated protein alpha chain antibody
CD 79a antibody
CD79a antibody
CD79a antigen (immunoglobulin-associated alpha) antibody
CD79A antigen antibody
CD79a molecule, immunoglobulin-associated alpha antibody
CD79A_HUMAN antibody
Ig alpha antibody
Ig-alpha antibody
ExpandImages
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Western blot analysis of CD79a on different lysates with Rabbit anti-CD79a antibody (HA722896) at 1/1,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: Daudi cell lysate
Lane 3: Raji cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 45-55 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722896) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Daudi cells labeling CD79a with Rabbit anti-CD79a antibody (HA722896) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD79a antibody (HA722896) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD79a antibody (HA722896) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722896) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-CD79a antibody (HA722896) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722896) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleenr tissue with Rabbit anti-CD79a antibody (HA722896) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722896) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/50
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CD79a Recombinant Rabbit Monoclonal Antibody [JE43-50]
Application: WB,IF-Tissue,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
CD79a Mouse Monoclonal Antibody [C1-E8]
Application: WB,FC
Reactivity: Human,Rat,Mouse
Conjugate: unconjugated
