Lin28A Recombinant Rabbit Monoclonal Antibody [JE60-36]
Catalog# HA722899
Lin28A Recombinant Rabbit Monoclonal Antibody [JE60-36]
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WB
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IF-Cell
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FC
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IP
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Human
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unconjugated
Overview
Product Name
Lin28A Recombinant Rabbit Monoclonal Antibody [JE60-36]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Lin28A aa 160-209 / 209.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC, IP
Molecular Weight
Predicted band size: 23 kDa
Positive Control
NCCIT cell lysate, JAR cell lysate, NCCIT.
Conjugation
unconjugated
Clone Number
JE60-36
Reactivity Data
| WB | IF-Cell | FC | IP | |
|---|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:500
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
LIN28A is conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs. LIN28A is localized to the periendoplasmic reticulum (ER) area and inhibits translation of mRNAs that are destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells.
Background References
1. Oyejobi GK et al. Regulating Protein-RNA Interactions: Advances in Targeting the LIN28/Let-7 Pathway. Int J Mol Sci. 2024 Mar.
2. Zhou F et al. Circular RNA circ_0096041 promotes osteosarcoma cell proliferation and migration via sponging miR-556-5p and regulating LIN28A expression. Cell Mol Biol (Noisy-le-grand). 2024 Feb.
Subcellular Location
Cytoplasm, Nucleus.
UNIPROT
Synonyms
CSDD1 antibody
FLJ12457 antibody
LIN 28 antibody
Lin 28 homolog A (C. elegans) antibody
Lin-28A antibody
LIN28 antibody
LIN28A antibody
LN28A_HUMAN antibody
Protein lin-28 homolog A antibody
ZCCHC1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Lin28A on different lysates with Rabbit anti-Lin28A antibody (HA722899) at 1/1,000 dilution.
Lane 1: NCCIT cell lysate
Lane 2: JAR cell lysate
Lane 3: HeLa cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 29 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722899) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of NCCIT (positive) and HeLa (negative) labeling Lin28A with Rabbit anti-Lin28A antibody (HA722899) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lin28A antibody (HA722899) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of NCCIT cells labeling Lin28A.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722899, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Lin28A was immunoprecipitated from 0.2 mg JAR cell lysate with HA722899 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722899 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: JAR cell lysate (input)
Lane 2: HA722899 IP in JAR cell lysate
Lane 3: Rabbit IgG instead of HA722899 in JAR cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 6 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"