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TEMT Recombinant Rabbit Monoclonal Antibody [JE64-74]

Usd: 385

Specification

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Catalog# HA722900

TEMT Recombinant Rabbit Monoclonal Antibody [JE64-74]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

TEMT Recombinant Rabbit Monoclonal Antibody [JE64-74]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TEMT aa 1-150 / 263.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 29 kDa

Positive Control

A549 cell lysate, Mouse liver tissue lysate, Mouse lung tissue lysate, Rat lung tissue lysate, Rat liver tissue lysate, human lung tissue, mouse lung tissue, rat lung tissue.

Conjugation

unconjugated

Clone Number

JE64-74

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Amine N-methyltransferase (EC 2.1.1.49), also called indolethylamine N-methyltransferase, and thioether S-methyltransferase, is an enzyme that is ubiquitously present in non-neural tissues and catalyzes the N-methylation of tryptamine and structurally related compounds. More recently, it was discovered that this enzyme can also catalyze the methylation of thioether and selenoether compounds, although the physiological significance of this biotransformation is not yet known.

Background References

1. Zhong S et al. Targeting INMT and interrupting its methylation pathway for the treatment of castration resistant prostate cancer. J Exp Clin Cancer Res. 2021 Sep

2. Glynos NG et al. Indolethylamine N-methyltransferase (INMT) is not essential for endogenous tryptamine-dependent methylation activity in rats. Sci Rep. 2023 Jan

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: O95050 Human

SwissProt: P40936 Mouse

Entrez Gene: 368066 Rat

Synonyms

Amine N methyltransferase antibody

Amine N-methyltransferase antibody

Aromatic alkylamine N methyltransferase antibody

Aromatic alkylamine N-methyltransferase antibody

Arylamine N methyltransferase antibody

Arylamine N-methyltransferase antibody

Indolamine N-methyltransferase antibody

Indolethylamine N methyltransferase antibody

Indolethylamine N-methyltransferase antibody

Inmt antibody

Expand

Images

  • Western blot analysis of TEMT on different lysates with Rabbit anti-TEMT antibody (<a href="/products/HA722900" style="font-weight: bold;text-decoration: underline;">HA722900</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate (20 µg/Lane)<br />Lane 2: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 3: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 4: Rat lung tissue lysate (40 µg/Lane)<br />Lane 5: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 29 kDa<br />Observed band size: 29 kDa<br /><br />Exposure time: Lane 1: 3 minutes; Lane 2-5: 42 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722900" style="font-weight: bold;text-decoration: underline;">HA722900</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TEMT on different lysates with Rabbit anti-TEMT antibody (HA722900) at 1/1,000 dilution.

    Lane 1: A549 cell lysate (20 µg/Lane)
    Lane 2: Mouse liver tissue lysate (40 µg/Lane)
    Lane 3: Mouse lung tissue lysate (40 µg/Lane)
    Lane 4: Rat lung tissue lysate (40 µg/Lane)
    Lane 5: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

    Exposure time: Lane 1: 3 minutes; Lane 2-5: 42 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722900) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-TEMT antibody (<a href="/products/HA722900" style="font-weight: bold;text-decoration: underline;">HA722900</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722900" style="font-weight: bold;text-decoration: underline;">HA722900</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-TEMT antibody (HA722900) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722900) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-TEMT antibody (<a href="/products/HA722900" style="font-weight: bold;text-decoration: underline;">HA722900</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722900" style="font-weight: bold;text-decoration: underline;">HA722900</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-TEMT antibody (HA722900) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722900) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-TEMT antibody (<a href="/products/HA722900" style="font-weight: bold;text-decoration: underline;">HA722900</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722900" style="font-weight: bold;text-decoration: underline;">HA722900</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-TEMT antibody (HA722900) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722900) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"