TEMT Recombinant Rabbit Monoclonal Antibody [JE64-74] (HA722900)
Catalog# HA722900
Rabbit Monoclonal to TEMT
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TEMT on different lysates with Rabbit anti-TEMT antibody (HA722900) at 1/1,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: Mouse liver tissue lysate (40 µg/Lane)
Lane 3: Mouse lung tissue lysate (40 µg/Lane)
Lane 4: Rat lung tissue lysate (40 µg/Lane)
Lane 5: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: Lane 1: 3 minutes; Lane 2-5: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722900) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: Mouse liver tissue lysate (40 µg/Lane)
Lane 3: Mouse lung tissue lysate (40 µg/Lane)
Lane 4: Rat lung tissue lysate (40 µg/Lane)
Lane 5: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: Lane 1: 3 minutes; Lane 2-5: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722900) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.