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Human Beta Nerve Growth Factor Recombinant Rabbit Monoclonal Antibody [PSH07-81] - BSA and Azide free (Detector)

Usd: 649

Specification

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Catalog# HA722904

Human Beta Nerve Growth Factor Recombinant Rabbit Monoclonal Antibody [PSH07-81] - BSA and Azide free (Detector)

Application

  • ELISA(Det)

Reactivity

  • Human

Pair: Capture
Pair: Detector
Pair: Protein
Range

  • 10.3-2,500 mg/mL

PI

  • 6.24

Conjugation

  • unconjugated

Overview

Product Name

Human Beta Nerve Growth Factor Recombinant Rabbit Monoclonal Antibody [PSH07-81] - BSA and Azide free (Detector)

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Beta Nerve Growth Factor aa 19-241 (HA211342).

Species Reactivity

Human

Validated Applications

ELISA(Det)

Positive Control

Recombinant Human Beta Nerve Growth Factor protein (HA211342).

Conjugation

unconjugated

Clone Number

PSH07-81

Product Features

Form

Liquid

Concentration

1 mg/mL(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • ELISA(Det)

  • Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-79] to Human Beta Nerve Growth Factor antibody (Capture) (HA722902) or Rabbit monoclonal [PSH07-80] to Human Beta Nerve Growth Factor antibody (Capture) (HA722903) and recombinant Human Human Beta Nerve Growth Factor protein (HA211342) as the standard. The reference range value is 10.3-2,500 mg/mL.

Target

Function

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed:20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form. A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN5 patients manifest loss of pain perception and impaired temperature sensitivity, ulcers, and in some cases self-mutilation. The autonomic involvement is variable.

Background References

1. Carvalho O.P., Thornton G.K., Hertecant J., Houlden H., Nicholas A.K., Cox J.J., Rielly M., Al-Gazali L., Woods C.G. A novel NGF mutation clarifies the molecular mechanism and extends the phenotypic spectrum of the HSAN5 neuropathy. J. Med. Genet. 48:131-135 (2011)

2. Wijeyewickrema L.C., Gardiner E.E., Gladigau E.L., Berndt M.C., Andrews R.K. Nerve growth factor inhibits metalloproteinase-disintegrins and blocks ectodomain shedding of platelet glycoprotein VI. J. Biol. Chem. 285:11793-11799 (2010)

Subcellular Location

Secreted

UNIPROT

SwissProt: P01138 Human

Synonyms

Beta nerve growth factor antibody

Beta NGF antibody

Beta-nerve growth factor antibody

Beta-NGF antibody

HSAN5 antibody

MGC161426 antibody

MGC161428 antibody

Nerve growth factor (beta polypeptide) antibody

Nerve growth factor antibody

Nerve growth factor beta antibody

Expand

Images

  • Sandwich ELISA analysis of human beta-NGF matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA722903" style="font-weight: bold;text-decoration: underline;">HA722903</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Beta Nerve Growth Factor protein (<a href="/products/HA211342" style="font-weight: bold;text-decoration: underline;">HA211342</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA722904" style="font-weight: bold;text-decoration: underline;">HA722904</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Sandwich ELISA analysis of human beta-NGF matched pair antibodies
    
    Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722903) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Beta Nerve Growth Factor protein (HA211342) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722904, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  • Sandwich ELISA analysis of human beta-NGF matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA722902" style="font-weight: bold;text-decoration: underline;">HA722902</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Beta Nerve Growth Factor protein (<a href="/products/HA211342" style="font-weight: bold;text-decoration: underline;">HA211342</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA722904" style="font-weight: bold;text-decoration: underline;">HA722904</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Sandwich ELISA analysis of human beta-NGF matched pair antibodies
    
    Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722902) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Beta Nerve Growth Factor protein (HA211342) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722904, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  • Interpolated concentrations of spiked beta-NGF in human cell culture media samples.<br /><br />The concentrations of beta-NGF were measured in duplicates, interpolated from the beta-NGF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    Interpolated concentrations of spiked beta-NGF in human cell culture media samples.
    
    The concentrations of beta-NGF were measured in duplicates, interpolated from the beta-NGF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated concentrations of spiked beta-NGF in human cell culture media samples.<br /><br />The concentrations of beta-NGF were measured in duplicates, interpolated from the beta-NGF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    Interpolated concentrations of spiked beta-NGF in human cell culture media samples.
    
    The concentrations of beta-NGF were measured in duplicates, interpolated from the beta-NGF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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