Mouse RANKL Recombinant Rabbit Monoclonal Antibody [PSH07-91] - BSA and Azide free (Capture)
Overview
Product Name
Mouse RANKL Recombinant Rabbit Monoclonal Antibody [PSH07-91] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse RANKL aa 158-316 (HA210558).
Species Reactivity
Mouse
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Mouse RANKL protein (HA210558).
Conjugation
unconjugated
Clone Number
PSH07-91
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-92] to Mouse RANKL antibody (Detector) (HA722924) and recombinant Mouse RANKL protein (HA210558) as the standard. The reference range value is 8.2-9,000pg/ml.
Target
Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca 2+ resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.
Background References
1. Mahoney D.J., Mikecz K., Ali T., Mabilleau G., Benayahu D., Plaas A., Milner C.M., Day A.J., Sabokbar A. TSG-6 regulates bone remodeling through inhibition of osteoblastogenesis and osteoclast activation. J. Biol. Chem. 283:25952-25962 (2008)
2. Decker C.E., Yang Z., Rimer R., Park-Min K.H., Macaubas C., Mellins E.D., Novack D.V., Faccio R. Tmem178 acts in a novel negative feedback loop targeting NFATc1 to regulate bone mass. Proc. Natl. Acad. Sci. U.S.A. 112:15654-15659 (2015)
Subcellular Location
Secreted.
UNIPROT
Synonyms
CD254 antibody
hRANKL2 antibody
ODF antibody
OPGL antibody
OPTB2 antibody
Osteoclast differentiation factor antibody
Osteoprotegerin ligand antibody
Rank Ligand antibody
RANKL antibody
Receptor activator of nuclear factor kappa B ligand antibody
ExpandImages
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Sandwich ELISA analysis of mouse RANKL matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722923) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse RANKL protein (HA210558) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA722924, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
☑ Cell treatment (CT)
Interpolated concentrations of native RANKL in mouse spleen cell culture supernatant treated or untreated with concanavalin A for 3days .
Interpolated concentration of native RANKL was measured in duplicate at different sample concentrations and interpolated from the RANKL standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean RANKL concentration was determined to be 3,455 pg/mL in neat mouse spleen cell treated supernatant, undetectable in untreated mouse spleen cell supernatant. -
Interpolated concentrations of spiked RANKL in cell culture media samples.
The concentrations of RANKL were measured in duplicates, interpolated from the RANKL standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"