Desmoglein 3 Recombinant Rabbit Monoclonal Antibody [JE40-30]
Catalog# HA722930
Desmoglein 3 Recombinant Rabbit Monoclonal Antibody [JE40-30]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Desmoglein 3 Recombinant Rabbit Monoclonal Antibody [JE40-30]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Desmoglein 3 aa 901-950 / 999.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 108 kDa
Positive Control
HaCaT cell lysate, A431 cell lysate, human esophagus tissue, mouse esophagus tissue, rat esophagus tissue.
Conjugation
unconjugated
Clone Number
JE40-30
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
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| Mouse |
|
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| Rat |
|
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
Desmoglein-3 is a protein that in humans is encoded by the DSG3 gene. In the skin epidermis Desmoglein-3 is expressed in the basal lower layers of the epidermis, and dominates in terms of expression on mucosal surfaces compared to Desmoglein-1. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein, along with Desmoglein-1, has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. The mucosal dominant form of pemphigus vulgaris only involves antibodies against Desmoglein-3 and causes mucosal erosions, but no skin lesions. Desmoglein-3 serves as a prognostic marker of Esophageal Squamous Cell Carcinoma (ESCC), and may even be involved in the progression of ESCC.
Background References
1. Takahashi H et al. T cell autoimmunity and immune regulation to desmoglein 3, a pemphigus autoantigen. J Dermatol. 2023 Feb
2. Ahmad US et al. Desmoglein-3 induces YAP phosphorylation and inactivation during collective migration of oral carcinoma cells. Mol Oncol. 2022 Apr
Subcellular Location
Cell membrane Curated, Cell junction, desmosome.
Synonyms
130 kD pemphigus vulgaris antigen antibody
130 kDa pemphigus vulgaris antigen antibody
Balding antibody
Cadherin family member 6 antibody
CDHF 6 antibody
CDHF6 antibody
Desmoglein 3 antibody
Desmoglein 3 precursor antibody
Desmoglein-3 antibody
Desmoglein3 antibody
ExpandImages
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Western blot analysis of Desmoglein 3 on different lysates with Rabbit anti-Desmoglein 3 antibody (HA722930) at 1/1,000 dilution.
Lane 1: HaCaT cell lysate
Lane 2: A431 cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 108 kDa
Observed band size: 140 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722930) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-Desmoglein 3 antibody (HA722930) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722930) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue with Rabbit anti-Desmoglein 3 antibody (HA722930) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722930) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-Desmoglein 3 antibody (HA722930) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722930) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"