Mouse Pro-Collagen I alpha 1 Recombinant Rabbit Monoclonal Antibody [PSH07-99] - BSA and Azide free (Detector)
Overview
Product Name
Mouse Pro-Collagen I alpha 1 Recombinant Rabbit Monoclonal Antibody [PSH07-99] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Pro-Collagen I alpha 1 protein aa aa 23-266 and 1083-1453.
Species Reactivity
Mouse
Validated Applications
ELISA(Det)
Positive Control
Recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113).
Conjugation
unconjugated
Clone Number
PSH07-99
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-98] to Mouse Pro-Collagen I alpha 1 antibody (Capture) (HA722934) and recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113) as the standard. The reference range value is 32.9-8,000 pg/ml.
Target
Function
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Background References
1. Korkko J.M., Ala-Kokko L., De Paepe A., Nuytinck L., Earley J.J., Prockop D.J. Analysis of the COL1A1 and COL1A2 genes by PCR amplification and scanning by conformation-sensitive gel electrophoresis identifies only COL1A1 mutations in 15 patients with osteogenesis imperfecta type I: identification of common sequences of null-allele mutations. Am. J. Hum. Genet. 62:98-110 (1998)
2. D\'Alessio M., Bernard M.P., Pretorius P.J., de Wet W., Ramirez F., Pretorious P.J. Complete nucleotide sequence of the region encompassing the first twenty- five exons of the human pro alpha 1(I) collagen gene (COL1A1). Gene 67:105-115 (1988)
Subcellular Location
Secreted.
UNIPROT
Synonyms
Alpha 1 type I collagen
Alpha-1 type I collagen
alpha1(I) procollagen
CO1A1_HUMAN
COL1A1
collagen 1
collagen alpha 1 chain type I
Collagen alpha-1(I) chain
collagen alpha-1(I) chain preproprotein
Collagen I alpha 1 polypeptide
ExpandImages
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![Sandwich ELISA analysis of mouse Pro-Collagen I alpha 1 matched pair antibodies<br /><br />Capture: <a href="/products/HA722934" style="font-weight: bold;text-decoration: underline;">HA722934</a>, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98]<br />Detector: <a href="/products/HA722935" style="font-weight: bold;text-decoration: underline;">HA722935</a>, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA722934" style="font-weight: bold;text-decoration: underline;">HA722934</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Pro-Collagen I alpha 1 protein (<a href="/products/HA211113" style="font-weight: bold;text-decoration: underline;">HA211113</a>) starting from 8000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA722935" style="font-weight: bold;text-decoration: underline;">HA722935</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA722935_1.png?v=20260429161819)
Sandwich ELISA analysis of mouse Pro-Collagen I alpha 1 matched pair antibodies
Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98]
Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722934) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Pro-Collagen I alpha 1 protein (HA211113) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA722935, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse samples.<br /><br />Capture: <a href="/products/HA722934" style="font-weight: bold;text-decoration: underline;">HA722934</a>, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98]<br />Detector: <a href="/products/HA722935" style="font-weight: bold;text-decoration: underline;">HA722935</a>, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99]<br /><br />Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 2146 ng/mL in mouse serum, 170 ng/ml in NIH/3T3 and 10 ng/ml mouse lung cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA722935_2.png?v=20260429161819)
Interpolated concentrations of native Pro-Collagen I alpha 1 in mouse samples.
Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98]
Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99]
Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 2146 ng/mL in mouse serum, 170 ng/ml in NIH/3T3 and 10 ng/ml mouse lung cell culture supernatant. -
![Interpolated concentrations of spiked Pro‑Collagen I alpha 1 in cell culture media samples.<br /><br />Capture: <a href="/products/HA722934" style="font-weight: bold;text-decoration: underline;">HA722934</a>, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98]<br />Detector: <a href="/products/HA722935" style="font-weight: bold;text-decoration: underline;">HA722935</a>, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99]<br /><br />The concentrations of Pro‑Collagen I alpha 1 were measured in duplicates, interpolated from the Pro‑Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA722935_3.png?v=20260429161819)
Interpolated concentrations of spiked Pro‑Collagen I alpha 1 in cell culture media samples.
Capture: HA722934, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-98]
Detector: HA722935, Mouse Pro-Collagen I alpha 1 Rabbit mAb [PSH07-99]
The concentrations of Pro‑Collagen I alpha 1 were measured in duplicates, interpolated from the Pro‑Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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