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HRP Conjugated 6X His tag Recombinant Rabbit Monoclonal Antibody [PSH07-10]

Usd: 409

Specification

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Catalog# HA722965

HRP Conjugated 6X His tag Recombinant Rabbit Monoclonal Antibody [PSH07-10]

Application

  • WB

  • IHC-P

Reactivity

  • Species independent

Conjugation

  • HRP

Overview

Product Name

HRP Conjugated 6X His tag Recombinant Rabbit Monoclonal Antibody [PSH07-10]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide immune sequence is HHHHHHHHC.

Species Reactivity

Species independent

Validated Applications

WB, IHC-P

Conjugation

HRP

Clone Number

PSH07-10

Reactivity Data

WB IHC-P
Species Independent
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:100,000

  • IHC-P

  • 1:50,000

Target

Function

His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Background References

暂无

Synonyms

6 His epitope tag antibody

Hexa His tag antibody

HHHHHH epitope tag antibody

HHHHHH tag antibody

His tag antibody

Polyhistidine Tag antibody

Images

  • Western blot analysis of 6X His tag on different lysates with Rabbit anti-6X His tag antibody (<a href="/products/HA722965" style="font-weight: bold;text-decoration: underline;">HA722965</a>) at 1/20,000 dilution.<br /><br />Lane 1: 293T transfected with His-tagged empty control cell lysate<br />Lane 2: 293T transfected with His-tagged ACAT2 (N-terminal) cell lysate<br />Lane 3: 293T transfected with His-tagged Histone H3.1 (C-terminal) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722965" style="font-weight: bold;text-decoration: underline;">HA722965</a>) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of 6X His tag on different lysates with Rabbit anti-6X His tag antibody (HA722965) at 1/20,000 dilution.

    Lane 1: 293T transfected with His-tagged empty control cell lysate
    Lane 2: 293T transfected with His-tagged ACAT2 (N-terminal) cell lysate
    Lane 3: 293T transfected with His-tagged Histone H3.1 (C-terminal) cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722965) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

  • Immunohistochemical analysis of paraffin-embedded HeLa transfected with His-tagged ACAT2 (N-terminal) cells with Rabbit anti-6X His tag antibody (<a href="/products/HA722965" style="font-weight: bold;text-decoration: underline;">HA722965</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722965" style="font-weight: bold;text-decoration: underline;">HA722965</a>) at 1/10,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded HeLa transfected with His-tagged ACAT2 (N-terminal) cells with Rabbit anti-6X His tag antibody (HA722965) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722965) at 1/10,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded HeLa transfected with His-tagged Histon H3.1 (C-terminal) cells with Rabbit anti-6X His tag antibody (<a href="/products/HA722965" style="font-weight: bold;text-decoration: underline;">HA722965</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722965" style="font-weight: bold;text-decoration: underline;">HA722965</a>) at 1/10,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded HeLa transfected with His-tagged Histon H3.1 (C-terminal) cells with Rabbit anti-6X His tag antibody (HA722965) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722965) at 1/10,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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