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Oxytocin-neurophysin 1 / OXT Recombinant Rabbit Monoclonal Antibody [PSH08-24]

Usd: 385

Specification

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Catalog# HA722968

Oxytocin-neurophysin 1 / OXT Recombinant Rabbit Monoclonal Antibody [PSH08-24]

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

Overview

Product Name

Oxytocin-neurophysin 1 / OXT Recombinant Rabbit Monoclonal Antibody [PSH08-24]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human OXT aa 1-125.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IHC-Fr, IF-Tissue

Target Molecular Weight

Predicted band size: 13 kDa

Positive Control

Mouse paraventricular nucleus tissue, rat supraoptic nucleus tissue, mouse pituitary tissue, rat pituitary tissue, Mouse pituitary tissue lysate, Rat pituitary tissue lysate.

Conjugation

unconjugated

Clone Number

PSH08-24

Reactivity Data

WB IHC-P IHC-Fr IF-Tissue
Human
Tested Tested
Mouse
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Cynomolgus Monkey
Published
Published
Published
Published
Pig
Published
Published
Published
Published

Product Features

Form

Liquid

Concentration

0.2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:2,000-1:5,000

  • IHC-Fr

  • 1:500-1:1,000

  • IF-Tissue

  • 1:2,000

Target

Function

Oxytocin is a peptide hormone and neuropeptide normally produced in the hypothalamus and released by the posterior pituitary. Present in animals since early stages of evolution, in humans it plays roles in behavior that include social bonding, love, reproduction, childbirth, and the period after childbirth. Oxytocin is released into the bloodstream as a hormone in response to sexual activity and during childbirth. It is also available in pharmaceutical form. In either form, oxytocin stimulates uterine contractions to speed up the process of childbirth. In its natural form, it also plays a role in maternal bonding and milk production. Production and secretion of oxytocin is controlled by a positive feedback mechanism, where its initial release stimulates production and release of further oxytocin. For example, when oxytocin is released during a contraction of the uterus at the start of childbirth, this stimulates production and release of more oxytocin and an increase in the intensity and frequency of contractions. This process compounds in intensity and frequency and continues until the triggering activity ceases. A similar process takes place during lactation and during sexual activity.

Background References

1. De Leon D et al. Methylation of OXT and OXTR genes, central oxytocin, and social behavior in female macaques. Horm Behav. 2020 Nov

2. Khazaal NM et al. The relationship between OXT gene polymorphisms and reproductive hormones in pregnant and lactating Awassi Ewes. Mol Biol Rep. 2023 Oct

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01178 Human

SwissProt: P35454 Mouse

Entrez Gene: 25504 Rat

Synonyms

MGC126890 antibody

MGC126892 antibody

Neurophysin 1 antibody

Neurophysin I antibody

Ocytocin antibody

OT antibody

OT NPI antibody

OXT antibody

Oxytocin antibody

Oxytocin Neurophysin preproprotein antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Paraventricular nucleus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Paraventricular nucleus

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Supraoptic nucleus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Supraoptic nucleus

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Pituitary <br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/2,000

    Application: IF-tissue

    Species: Mouse

    Site: Pituitary

    Sample: Paraffin-embedded section

    Antibody concentration: 1/2,000

  • Application: IF-tissue<br /><br />Species: Rat<br /><br />Site: Pituitary <br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/2,000

    Application: IF-tissue

    Species: Rat

    Site: Pituitary

    Sample: Paraffin-embedded section

    Antibody concentration: 1/2,000

  • Immunohistochemical analysis of paraffin-embedded mouse paraventricular nucleus tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse paraventricular nucleus tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722968) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722968) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue (negative) with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue (negative) with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722968) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722968) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue (negative) with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue (negative) with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722968) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of Oxytocin-neurophysin 1 / OXT on different lysates with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse pituitary tissue lysate<br />Lane 2: Rat pituitary tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 13 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722968" style="font-weight: bold;text-decoration: underline;">HA722968</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Oxytocin-neurophysin 1 / OXT on different lysates with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA722968) at 1/1,000 dilution.

    Lane 1: Mouse pituitary tissue lysate
    Lane 2: Rat pituitary tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 13 kDa
    Observed band size: 14 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722968) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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