Biotin Conjugated Mouse IL-33 Recombinant Rabbit Monoclonal Antibody [PSH08-42] - Detector
Overview
Product Name
Biotin Conjugated Mouse IL-33 Recombinant Rabbit Monoclonal Antibody [PSH08-42] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse IL-33 aa 109-266 (HA210768).
Species Reactivity
Mouse
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse IL-33 protein (HA210768).
Conjugation
Biotin
Clone Number
PSH08-42
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-41] to Mouse IL-33 antibody (Capture) (HA722985) and recombinant Mouse IL-33 protein (HA210768) as the standard. The reference range value is 15.6-2,000 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
Target
Function
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as an enhancer of polarization of alternatively activated macrophages. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury. Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets.
Background References
1. Faas M., Ipseiz N., Ackermann J., Culemann S., Grueneboom A., Schroeder F., Rothe T. IL-33-induced metabolic reprogramming controls the differentiation of alternatively activated macrophages and the resolution of inflammation. Immunity 54:2531-2546.e5 (2021)
2. Osbourn M., Soares D.C., Vacca F., Cohen E.S., Scott I.C., Gregory W.F., Smyth D.J. HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33. Immunity 47:739-751 (2017)
Subcellular Location
Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted.
UNIPROT
Synonyms
C9orf26
CHROMOSOME 9 OPEN READING FRAME 26
DKFZp586H0523
DVS27
DVS27 related protein
IL 1F11
IL 33
IL-1F11
IL-33
IL1F11
ExpandImages
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Sandwich ELISA analysis of mouse IL-33 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722985) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722987B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Sandwich ELISA analysis of mouse IL-33 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722985) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-33 protein (HA210768) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722987B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"