Human CCL25 Recombinant Rabbit Monoclonal Antibody [PSH08-67] - BSA and Azide free (Detector)
Overview
Product Name
Human CCL25 Recombinant Rabbit Monoclonal Antibody [PSH08-67] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CCL25 aa 24-150 (HA211000).
Species Reactivity
Human
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human CCL25 protein (HA211000).
Conjugation
unconjugated
Clone Number
PSH08-67
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-65] to Human CCL25 antibody (Capture) (HA723015) and recombinant Human CCL25 protein (HA211000) as the standard. The reference range value is 20.6-5,000 pg/mL.
Target
Function
This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. The product of this gene binds to chemokine receptor CCR9. Alternative splicing results in multiple transcript variants. Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Background References
1. Vicari A.P., Figueroa D.J., Hedrick J.A., Foster J.S., Singh K.P., Menon S., Copeland N.G., Gilbert D.J., Jenkins N.A., Zlotnik A. TECK: a novel CC chemokine specifically expressed by thymic dendritic cells and potentially involved in T cell development. Immunity 7:291-301 (1997)
2. Zaballos A., Gutierrez J., Varona R., Ardavin C., Marquez G. Identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. J. Immunol. 162:5671-5675 (1999)
Subcellular Location
Secreted.
UNIPROT
Synonyms
A130072A22Rik antibody
C-C motif chemokine 25 antibody
CCL 25 antibody
CCL25 antibody
CCL25_HUMAN antibody
Chemokine (C C motif) ligand 25 antibody
Chemokine (CC motif) ligand 25 antibody
Chemokine C C motif ligand 25 antibody
Chemokine CC motif ligand 25 antibody
Chemokine TECK antibody
ExpandImages
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Sandwich ELISA analysis of Human CCL25 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723015) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL25 protein (HA211000) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA723017, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of spiked TECK in cell culture media samples.
The concentrations of TECK were measured in duplicates, interpolated from the TECK standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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