DNA polymerase mu Recombinant Rabbit Monoclonal Antibody [PSH08-75]

Function

DNA polymerase mu is a polymerase enzyme found in eukaryotes. In humans, this protein is encoded by the POLM gene. Pol μ is a member of the X family of DNA polymerases. It participates in resynthesis of damaged or missing nucleotides during the non-homologous end joining (NHEJ) pathway of DNA repair. Pol μ interacts with Ku and DNA ligase IV, which also participate in NHEJ. It is structurally and functionally related to pol λ, and, like pol λ, pol μ has a BRCT domain that is thought to mediate interactions with other DNA repair proteins. Unlike pol λ, however, pol μ has the unique ability to add a base to a blunt end that is templated by the overhang on the opposite end of the double-strand break. Pol μ is also closely related to terminal deoxynucleotidyl transferase (TdT), a specialized DNA polymerase that adds random nucleotides to DNA ends during V(D)J recombination, the process by which B-cell and T-cell receptor diversity is generated in the vertebrate immune system. Like TdT, pol μ participates in V(D)J recombination, but only during light chain rearrangements. This is distinct from pol λ, which is involved in heavy chain rearrangements.

Background References

1. Kaminski AM et al. DNA polymerase mu: An inflexible scaffold for substrate flexibility. DNA Repair (Amst). 2020 Sep

2. Guo M et al. Mechanism of genome instability mediated by human DNA polymerase mu misincorporation. Nat Commun. 2021 Jun

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q9NP87 Human

Synonyms