Biotin Conjugated Mouse/Rat Leptin Recombinant Rabbit Monoclonal Antibody [PSH08-95] - Detector
Overview
Product Name
Biotin Conjugated Mouse/Rat Leptin Recombinant Rabbit Monoclonal Antibody [PSH08-95] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Leptin aa 22-167.
Species Reactivity
Mouse, Rat
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse Leptin protein (HA210898).
Conjugation
Biotin
Clone Number
PSH08-95
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH08-94] to Mouse/Rat Leptin antibody (Capture) (HA723046) and recombinant Mouse/Rat Leptin protein (HA210898) as the standard. The reference range value is 31-4,000 pg/ml.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways. In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity. In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides. In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption. Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1.
Background References
1. Bates S.H., Stearns W.H., Dundon T.A., Schubert M., Tso A.W., Wang Y., Banks A.S., Lavery H.J., Haq A.K., Maratos-Flier E., Neel B.G., Schwartz M.W., Myers M.G. Jr. STAT3 signalling is required for leptin regulation of energy balance but not reproduction. Nature 421:856-859 (2003)
2. Ramadori G., Fujikawa T., Fukuda M., Anderson J., Morgan D.A., Mostoslavsky R., Stuart R.C., Perello M., Vianna C.R., Nillni E.A., Rahmouni K., Coppari R. SIRT1 deacetylase in POMC neurons is required for homeostatic defenses against diet-induced obesity. Cell Metab. 12:78-87 (2010)
Subcellular Location
Secreted.
Synonyms
FLJ94114 antibody
LEP antibody
LEP_HUMAN antibody
LEPD antibody
Leptin (murine obesity homolog) antibody
Leptin (obesity homolog, mouse) antibody
Leptin antibody
Leptin Murine Obesity Homolog antibody
Leptin Precursor Obesity Factor antibody
OB antibody
ExpandImages
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Sandwich ELISA analysis of Mouse/Rat leptin matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723046) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Leptin protein (HA210898) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723047, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native leptin in mouse serum and rat serum samples.
The concentrations of leptin were interpolated from the leptin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 25% and rat serum 25%. The mean leptin concentration was determined to be 9,628 pg/ml in mouse serum and 2,283 pg/ml in rat serum. -
Interpolated concentrations of spiked leptin in mouse cell culture media samples.
The concentrations of leptin were measured in duplicates, interpolated from the leptin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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