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Cystatin F Recombinant Rabbit Monoclonal Antibody [PSH09-00]

Usd: 385

Specification

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Catalog# HA723053

Cystatin F Recombinant Rabbit Monoclonal Antibody [PSH09-00]

Application

  • WB

  • IHC-Fr

Reactivity

  • Human

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Cystatin F Recombinant Rabbit Monoclonal Antibody [PSH09-00]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Cystatin F aa 1-145.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-Fr

Molecular Weight

Predicted band size: 16 kDa

Positive Control

U-937 cell lysate, mouse spleen tissue.

Conjugation

unconjugated

Clone Number

PSH09-00

Reactivity Data

WB IHC-Fr
Human
Tested Tested
Mouse
Not Recommended
Tested Tested
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-Fr

  • 1:100

Target

Function

Cystatin-F is a protein that in humans is encoded by the CST7 gene. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immune regulation through inhibition of a unique target in the hematopoietic system. Expression of the protein has been observed in various human cancer cell lines established from malignant tumors.

Background References

1. Ni J, Fernandez MA, Danielsson L, Chillakuru RA, Zhang J, Grubb A, Su J, Gentz R, Abrahamson M (Oct 1998). "Cystatin F is a glycosylated human low molecular weight cysteine proteinase inhibitor". J Biol Chem. 273 (38): 24797–804. doi:10.1074/jbc.273.38.24797. PMID 9733783.

2. Halfon S, Ford J, Foster J, Dowling L, Lucian L, Sterling M, Xu Y, Weiss M, Ikeda M, Liggett D, Helms A, Caux C, Lebecque S, Hannum C, Menon S, McClanahan T, Gorman D, Zurawski G (Aug 1998). "Leukocystatin, a new Class II cystatin expressed selectively by hematopoietic cells". J Biol Chem. 273 (26): 16400–8. doi:10.1074/jbc.273.26.16400. PMID 9632704.

Subcellular Location

Secreted, Cytoplasm.

UNIPROT

SwissProt: O76096 Human

SwissProt: O89098 Mouse

Synonyms

CMAP antibody

CST 7 antibody

Cst7 antibody

Cystatin 7 antibody

cystatin F (leukocystatin) antibody

Cystatin like metastasis associated protein antibody

Cystatin-7 antibody

Cystatin-F antibody

Cystatin-like metastasis-associated protein antibody

Cystatin7 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Cystatin F on different lysates with Rabbit anti-Cystatin F antibody (<a href="/products/HA723053" style="font-weight: bold;text-decoration: underline;">HA723053</a>) at 1/2,000 dilution.<br /><br />Lane 1: U-937 cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (negative) (20 µg/Lane)<br /><br />Predicted band size: 16 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723053" style="font-weight: bold;text-decoration: underline;">HA723053</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Cystatin F on different lysates with Rabbit anti-Cystatin F antibody (HA723053) at 1/2,000 dilution.

    Lane 1: U-937 cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (negative) (20 µg/Lane)

    Predicted band size: 16 kDa
    Observed band size: 20 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723053) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-Cystatin F antibody (<a href="/products/HA723053" style="font-weight: bold;text-decoration: underline;">HA723053</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA723053" style="font-weight: bold;text-decoration: underline;">HA723053</a>, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-Cystatin F antibody (HA723053) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723053, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunofluorescence analysis of frozen mouse brain tissue (negative) with Rabbit anti-Cystatin F antibody (<a href="/products/HA723053" style="font-weight: bold;text-decoration: underline;">HA723053</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA723053" style="font-weight: bold;text-decoration: underline;">HA723053</a>, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    ☑ Relative expression (RE)

    Immunofluorescence analysis of frozen mouse brain tissue (negative) with Rabbit anti-Cystatin F antibody (HA723053) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723053, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"