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Biotin Conjugated Human CCL23 Recombinant Rabbit Monoclonal Antibody [PSH09-07] - BSA and Azide free (Detector)

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Specification

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Catalog# HA723064B

Biotin Conjugated Human CCL23 Recombinant Rabbit Monoclonal Antibody [PSH09-07] - BSA and Azide free (Detector)

Application

  • ELISA(Det)

  • ELISA

Reactivity

  • Human

Pair: Capture
Pair: Detector
Pair: Protein
Range

  • 8-2,000 pg/mL

PI

  • 6.8

Conjugation

  • Biotin

Overview

Product Name

Biotin Conjugated Human CCL23 Recombinant Rabbit Monoclonal Antibody [PSH09-07] - BSA and Azide free (Detector)

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human CCL23 aa 22-120.

Species Reactivity

Human

Validated Applications

ELISA(Det), ELISA

Positive Control

Recombinant Human CCL23 protein (HA210894).

Conjugation

Biotin

Clone Number

PSH09-07

Product Features

Form

Liquid

Concentration

1 mg/ml.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • ELISA(Det)

  • Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-05] to Human CCL23 antibody (Capture) (HA723061) or Rabbit monoclonal [PSH09-06] to Human CCL23 antibody (Capture) (HA723062) and Recombinant Human CCL23 protein (HA210894) as the standard. The reference range value is 8-2,000 pg/mL.

  • ELISA

  • Use at an assay dependent concentration.

Target

Function

Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than CCL23. This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. In addition, the product of this gene is a potent agonist of the chemokine (C-C motif) receptor 1. Alternative splicing results in multiple transcript variants that encode different isoforms.

Background References

1. Berahovich R.D., Miao Z., Wang Y., Premack B., Howard M.C., Schall T.J. Proteolytic activation of alternative CCR1 ligands in inflammation. J. Immunol. 174:7341-7351 (2005)

Subcellular Location

Secreted.

UNIPROT

SwissProt: P55773 Human

Synonyms

C C motif chemokine ligand 23 antibody

C-C motif chemokine 23 antibody

C6 beta chemokine antibody

CCL 23 antibody

CCL23 antibody

CCL23(30-99) antibody

CCL23_HUMAN antibody

Chemokine (C C motif) ligand 23 antibody

Chemokine CC motif ligand 23 antibody

ck beta 8 antibody

Expand

Images

  • Sandwich ELISA analysis of human Von Willebrand Factor matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723062" style="font-weight: bold;text-decoration: underline;">HA723062</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human CCL23 (<a href="/products/HA210894" style="font-weight: bold;text-decoration: underline;">HA210894</a>) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723064B" style="font-weight: bold;text-decoration: underline;">HA723064B</a>, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Sandwich ELISA analysis of human Von Willebrand Factor matched pair antibodies
    
    Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723062) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human CCL23 (HA210894) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723064B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  • Sandwich ELISA analysis of Human CCL23 matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723061" style="font-weight: bold;text-decoration: underline;">HA723061</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human CCL23 (<a href="/products/HA210894" style="font-weight: bold;text-decoration: underline;">HA210894</a>) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723064B" style="font-weight: bold;text-decoration: underline;">HA723064B</a>, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Sandwich ELISA analysis of Human CCL23 matched pair antibodies
    
    Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723061) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human CCL23 (HA210894) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723064B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  • Interpolated concentrations of native CCL23 in human serum samples.<br /><br />The concentrations of CCL23 were measured in duplicates, interpolated from the CCL23 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL23 concentration was determined to be 679.6 pg/ml in human serum.

    Interpolated concentrations of native CCL23 in human serum samples.
    
    The concentrations of CCL23 were measured in duplicates, interpolated from the CCL23 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL23 concentration was determined to be 679.6 pg/ml in human serum.

  • Interpolated concentrations of native CCL23 in human serum samples.<br /><br />The concentrations of CCL23 were measured in duplicates, interpolated from the CCL23 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL23 concentration was determined to be 673.4 pg/ml in human serum.

    Interpolated concentrations of native CCL23 in human serum samples.
    
    The concentrations of CCL23 were measured in duplicates, interpolated from the CCL23 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL23 concentration was determined to be 673.4 pg/ml in human serum.

  • Interpolated concentrations of spiked CCL23 in human cell culture media samples.<br /><br />The concentrations of CCL23 were interpolated from the CCL23 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.

    Interpolated concentrations of spiked CCL23 in human cell culture media samples.
    
    The concentrations of CCL23 were interpolated from the CCL23 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.

  • Interpolated concentrations of spiked CCL23 in human cell culture media samples.<br /><br />The concentrations of CCL23 were measured in duplicates, interpolated from the CCL23 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    Interpolated concentrations of spiked CCL23 in human cell culture media samples.
    
    The concentrations of CCL23 were measured in duplicates, interpolated from the CCL23 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"