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SIRT7 Recombinant Rabbit Monoclonal Antibody [PSH09-17]

Usd: 385

Specification

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Catalog# HA723075

SIRT7 Recombinant Rabbit Monoclonal Antibody [PSH09-17]

Application

  • WB

  • IF-Cell

  • IHC-P

  • IP

  • ChIP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SIRT7 Recombinant Rabbit Monoclonal Antibody [PSH09-17]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SIRT7 aa 1-400.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, IP, ChIP

Molecular Weight

Predicted band size: 45 kDa

Positive Control

HEK-293 cell lysate, HeLa cell lysate, U-2 OS cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse liver tissue lysate, Rat spleen tissue lysate, RAW264.7, mouse liver tissue.

Conjugation

unconjugated

Clone Number

PSH09-17

Reactivity Data

WB IF-Cell IHC-P IP ChIP
Human
Tested Tested
Tested
Not Recommended
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:500

  • IP

  • 1-2μg/sample

  • ChIP

  • Use 0.5~2 μg for 25 μg of chromatin.

Target

Function

NAD-dependent deacetylase sirtuin 7 is an enzyme that in humans is encoded by the SIRT7 gene. SIRT7 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. SIRT7 facilitates the transcription of DNA by DNA polymerase I, DNA polymerase II, and DNA polymerase III. In human cells, SIRT7 has only been shown to interact with two other molecules: RNA polymerase I (RNA Pol I) and upstream binding factor (UBF). SIRT7 is localized to the nucleolus and interacts with RNA Pol I. Chromatin immunoprecipitation studies demonstrate that SIRT7 localizes to rDNA, and coimmunoprecipitation shows that SIRT7 binds RNA Pol I.

Background References

1. Raza U et al. SIRT7: the seventh key to unlocking the mystery of aging. Physiol Rev. 2024 Jan

2. Yamagata K et al. The Emerging Role of SIRT7 in Glucose and Lipid Metabolism. Cells. 2023 Dec

Subcellular Location

Nucleus, nucleolus, nucleoplasm, Chromosome, Cytoplasm.

UNIPROT

SwissProt: Q9NRC8 Human

SwissProt: Q8BKJ9 Mouse

SwissProt: B2RZ55 Rat

Synonyms

NAD dependent deacetylase sirtuin 7 antibody

NAD dependent protein deacetylase sirtuin 7 antibody

NAD-dependent protein deacetylase sirtuin-7 antibody

Regulatory protein SIR2 homolog 7 antibody

Silent mating type information regulation 2 homolog antibody

Silent mating type information regulation 2 S.cerevisiae homolog 7 antibody

SIR2 L7 antibody

SIR2 like protein 7 antibody

Sir2 related protein type 7 antibody

SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7 antibody

Expand

Images

  • Western blot analysis of SIRT7 on different lysates with Rabbit anti-SIRT7 antibody (<a href="/products/HA723075" style="font-weight: bold;text-decoration: underline;">HA723075</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: U-2 OS cell lysate<br />Lane 4: Neuro-2a cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: Mouse liver tissue lysate<br />Lane 7: Rat spleen tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723075" style="font-weight: bold;text-decoration: underline;">HA723075</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SIRT7 on different lysates with Rabbit anti-SIRT7 antibody (HA723075) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: U-2 OS cell lysate
    Lane 4: Neuro-2a cell lysate
    Lane 5: C6 cell lysate
    Lane 6: Mouse liver tissue lysate
    Lane 7: Rat spleen tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723075) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of RAW264.7 cells labeling SIRT7 with Rabbit anti-SIRT7 antibody (<a href="/products/HA723075" style="font-weight: bold;text-decoration: underline;">HA723075</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIRT7 antibody (<a href="/products/HA723075" style="font-weight: bold;text-decoration: underline;">HA723075</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of RAW264.7 cells labeling SIRT7 with Rabbit anti-SIRT7 antibody (HA723075) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIRT7 antibody (HA723075) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SIRT7 antibody (<a href="/products/HA723075" style="font-weight: bold;text-decoration: underline;">HA723075</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723075" style="font-weight: bold;text-decoration: underline;">HA723075</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SIRT7 antibody (HA723075) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723075) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SIRT7 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA723075" style="font-weight: bold;text-decoration: underline;">HA723075</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723075" style="font-weight: bold;text-decoration: underline;">HA723075</a> at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA723075" style="font-weight: bold;text-decoration: underline;">HA723075</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723075" style="font-weight: bold;text-decoration: underline;">HA723075</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 30 seconds; ECL: <a href="/products/K1802" style="font-weight: bold;text-decoration: underline;">K1802</a>

    SIRT7 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723075 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723075 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA723075 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA723075 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 30 seconds; ECL: K1802

  • Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with SIRT7 (<a href="/products/HA723075" style="font-weight: bold;text-decoration: underline;">HA723075</a>) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

    Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with SIRT7 (HA723075) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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