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Aquaporin 2 Recombinant Rabbit Monoclonal Antibody [PSH09-19]

Usd: 385

Specification

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Catalog# HA723077

Aquaporin 2 Recombinant Rabbit Monoclonal Antibody [PSH09-19]

Application

  • IHC-P

  • IF-Tissue

  • IHC-Fr

  • mIHC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Aquaporin 2 Recombinant Rabbit Monoclonal Antibody [PSH09-19]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Aquaporin 2 aa 215-263/271

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P, IF-Tissue, IHC-Fr, mIHC

Target Molecular Weight

Predicted band size: 29 kDa

Positive Control

Mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

PSH09-19

Reactivity Data

IHC-P IF-Tissue IHC-Fr mIHC
Human
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:100,000

  • IHC-Fr

  • 1:1,000-1:10,000

  • IF-Tissue

  • 1:5,000

  • mIHC

  • 1:2,000

Target

Function

Aquaporin-2 (AQP-2) is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. It is encoded by the AQP2 gene.It is the only aquaporin regulated by vasopressin.The basic job of aquaporin 2 is to reabsorb water from the urine while its being removed from the blood by the kidney. Aquaporin 2 is in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed, vasopressin binds to the cell surface vasopressin receptor thereby activating a signaling pathway that causes the aquaporin 2 containing vesicles to fuse with the plasma membrane, so the aquaporin 2 can be used by the cell.

Background References

1. Valenti G, Tamma G. The vasopressin-aquaporin-2 pathway syndromes. Handb Clin Neurol. 2021;181:249-259. doi: 10.1016/B978-0-12-820683-6.00018-X. PMID: 34238461.

2. Olesen ET, Fenton RA. Aquaporin-2 membrane targeting: still a conundrum. Am J Physiol Renal Physiol. 2017 Apr 1;312(4):F744-F747. doi: 10.1152/ajprenal.00010.2017. Epub 2017 Feb 8. PMID: 28179252.

Subcellular Location

Apical cell membrane, Basolateral cell membrane, Cell membrane, Cytoplasmic vesicle membrane, Golgi apparatus, trans-Golgi network membrane

UNIPROT

SwissProt: P41181 Human

SwissProt: P56402 Mouse

SwissProt: P34080 Rat

Synonyms

ADH water channel antibody

AQP 2 antibody

AQP CD antibody

AQP-2 antibody

AQP-CD antibody

AQP2 antibody

AQP2_HUMAN antibody

AQPCD antibody

Aquaporin 2 collecting duct antibody

Aquaporin CD antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: kidney<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/5,000<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: kidney

    Sample: Frozen section

    Antibody concentration: 1/5,000

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: kidney<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/1,000<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: kidney

    Sample: Frozen section

    Antibody concentration: 1/1,000

    Antigen retrieval: Not required

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: kidney<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/5,000

    Application: IF-Tissue

    Species: Mouse

    Site: kidney

    Sample: Paraffin-embedded section

    Antibody concentration: 1/5,000

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Aquaporin 2 antibody (<a href="/products/HA723077" style="font-weight: bold;text-decoration: underline;">HA723077</a>) at 1/100,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723077" style="font-weight: bold;text-decoration: underline;">HA723077</a>) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Aquaporin 2 antibody (HA723077) at 1/100,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723077) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse colon tissue (negative) with Rabbit anti-Aquaporin 2 antibody (<a href="/products/HA723077" style="font-weight: bold;text-decoration: underline;">HA723077</a>) at 1/100,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723077" style="font-weight: bold;text-decoration: underline;">HA723077</a>) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue (negative) with Rabbit anti-Aquaporin 2 antibody (HA723077) at 1/100,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723077) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Aquaporin 2 antibody (<a href="/products/HA723077" style="font-weight: bold;text-decoration: underline;">HA723077</a>) at 1/100,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723077" style="font-weight: bold;text-decoration: underline;">HA723077</a>) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Aquaporin 2 antibody (HA723077) at 1/100,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723077) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat colon tissue (negative) with Rabbit anti-Aquaporin 2 antibody (<a href="/products/HA723077" style="font-weight: bold;text-decoration: underline;">HA723077</a>) at 1/100,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723077" style="font-weight: bold;text-decoration: underline;">HA723077</a>) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue (negative) with Rabbit anti-Aquaporin 2 antibody (HA723077) at 1/100,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723077) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"