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RBMX2 Recombinant Rabbit Monoclonal Antibody [PSH09-47]

Usd: 385

Specification

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Catalog# HA723109

RBMX2 Recombinant Rabbit Monoclonal Antibody [PSH09-47]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

RBMX2 Recombinant Rabbit Monoclonal Antibody [PSH09-47]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human RBMX2 aa 1-322/322

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 37 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, A431 cell lysate, HepG2 cell lysate, MCF7 cell lysate.

Conjugation

unconjugated

Clone Number

PSH09-47

Reactivity Data

WB
Human
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

RBMX is a ubiquitously expressed nuclear RNA binding protein that is encoded by a gene on the X chromosome. RBMX belongs to a small protein family with additional members encoded by paralogs on the mammalian Y chromosome and other chromosomes. These RNA binding proteins are important for normal development, and also implicated in cancer and viral infection. At the molecular level RBMX family proteins contribute to splicing control, transcription and genome integrity.

Background References

1. Zhi Li,Junlong Liu,Shuaiyang Zhao, et al. Theileria annulata Subtelomere-Encoded Variable Secreted Protein-TA05575 Binds to Bovine RBMX2. Frontiers in Cellular and Infection Microbiology. 2021;11 (0):0-0. doi:10.3389/fcimb.2021.644983

2. Pia Laine,William J. Rowell,Lars Paulín, et al. Alu element in the RNA binding motif protein, X-linked 2 (RBMX2) gene found to be linked to bipolar disorder. PLOS ONE. 2021;16 (12):e0261170-e0261170. doi:10.1371/journal.pone.0261170

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q9Y388 Human

Synonyms

CGI-79 antibody

Rbmx2 antibody

RBMX2_HUMAN antibody

RNA binding motif protein X linked 2 antibody

RNA-binding motif protein antibody

X-linked 2 antibody

Images

  • Western blot analysis of RBMX2 on different lysates with Rabbit anti-RBMX2 antibody (<a href="/products/HA723109" style="font-weight: bold;text-decoration: underline;">HA723109</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate <br />Lane 2: A549 cell lysate <br />Lane 3: A431 cell lysate <br />Lane 4: HepG2 cell lysate <br />Lane 5: MCF7 cell lysate <br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 37 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723109" style="font-weight: bold;text-decoration: underline;">HA723109</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RBMX2 on different lysates with Rabbit anti-RBMX2 antibody (HA723109) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate
    Lane 3: A431 cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: MCF7 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723109) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of RBMX2 on different lysates with Rabbit anti-RBMX2 antibody (<a href="/products/HA723109" style="font-weight: bold;text-decoration: underline;">HA723109</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa-si NT cell lysate<br />Lane 2: HeLa-si RBMX2 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 37 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723109" style="font-weight: bold;text-decoration: underline;">HA723109</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of RBMX2 on different lysates with Rabbit anti-RBMX2 antibody (HA723109) at 1/2,000 dilution.

    Lane 1: HeLa-si NT cell lysate
    Lane 2: HeLa-si RBMX2 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 37 kDa
    Observed band size: 45 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723109) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"