RBMX2 Recombinant Rabbit Monoclonal Antibody [PSH09-47]
Catalog# HA723109
RBMX2 Recombinant Rabbit Monoclonal Antibody [PSH09-47]
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WB
-
Human
-
unconjugated
Overview
Product Name
RBMX2 Recombinant Rabbit Monoclonal Antibody [PSH09-47]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human RBMX2 aa 1-322/322
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 37 kDa
Positive Control
HeLa cell lysate, A549 cell lysate, A431 cell lysate, HepG2 cell lysate, MCF7 cell lysate.
Conjugation
unconjugated
Clone Number
PSH09-47
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
Target
Function
RBMX is a ubiquitously expressed nuclear RNA binding protein that is encoded by a gene on the X chromosome. RBMX belongs to a small protein family with additional members encoded by paralogs on the mammalian Y chromosome and other chromosomes. These RNA binding proteins are important for normal development, and also implicated in cancer and viral infection. At the molecular level RBMX family proteins contribute to splicing control, transcription and genome integrity.
Background References
1. Zhi Li,Junlong Liu,Shuaiyang Zhao, et al. Theileria annulata Subtelomere-Encoded Variable Secreted Protein-TA05575 Binds to Bovine RBMX2. Frontiers in Cellular and Infection Microbiology. 2021;11 (0):0-0. doi:10.3389/fcimb.2021.644983
2. Pia Laine,William J. Rowell,Lars Paulín, et al. Alu element in the RNA binding motif protein, X-linked 2 (RBMX2) gene found to be linked to bipolar disorder. PLOS ONE. 2021;16 (12):e0261170-e0261170. doi:10.1371/journal.pone.0261170
Subcellular Location
Nucleus.
UNIPROT
Synonyms
CGI-79 antibody
Rbmx2 antibody
RBMX2_HUMAN antibody
RNA binding motif protein X linked 2 antibody
RNA-binding motif protein antibody
X-linked 2 antibody
Images
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Western blot analysis of RBMX2 on different lysates with Rabbit anti-RBMX2 antibody (HA723109) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: A431 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723109) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of RBMX2 on different lysates with Rabbit anti-RBMX2 antibody (HA723109) at 1/2,000 dilution.
Lane 1: HeLa-si NT cell lysate
Lane 2: HeLa-si RBMX2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 45 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723109) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"