CCS Recombinant Rabbit Monoclonal Antibody [PSH09-50]
Catalog# HA723112
CCS Recombinant Rabbit Monoclonal Antibody [PSH09-50]
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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HA751297
不含抗保成分
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unconjugated
Overview
Product Name
CCS Recombinant Rabbit Monoclonal Antibody [PSH09-50]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CCS aa 1-274.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Molecular Weight
Predicted band size: 29 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, Jurkat cell lysate, 293T cell lysate, LNCaP cell lysate, MCF7 cell lysate, A549 cell lysate, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue.
Conjugation
unconjugated
Clone Number
PSH09-50
Reactivity Data
| WB | IHC-P | IP | |
|---|---|---|---|
| Human |
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|
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| Mouse |
|
|
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| Rat |
|
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:200
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IP
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1-2μg/sample
Target
Function
Copper chaperone for superoxide dismutase is a metalloprotein that is responsible for the delivery of Cu to superoxide dismutase (SOD1). CCS is present in mammals and most eukaryotes including yeast. The structure of CCS is composed of three distinct domains that are necessary for its function. Although CCS is important for many organisms, there are CCS independent pathways for SOD1, and many species lack CCS all together, such as C. elegans. In humans the protein is encoded by the CCS gene.
Background References
1. Zhang T et al. Copper Chaperone for Superoxide Dismutase FoCCS1 in Frankliniella occidentalis May Be Associated with Feeding Adaptation after Host Shifting. Insects. 2022 Aug
Subcellular Location
Cytoplasm.
Synonyms
CCS antibody
CCS_HUMAN antibody
Copper chaperone for superoxide dismutase antibody
MGC138260 antibody
SOD 4 antibody
SOD4 antibody
Superoxide dismutase copper chaperone antibody
Images
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Western blot analysis of CCS on different lysates with Rabbit anti-CCS antibody (HA723112) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: 293T cell lysate
Lane 5: LNCaP cell lysate
Lane 6: MCF7 cell lysate
Lane 7: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 33 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723112) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CCS antibody (HA723112) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723112) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CCS antibody (HA723112) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723112) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-CCS antibody (HA723112) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723112) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-CCS antibody (HA723112) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723112) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
CCS was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723112 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723112 at 1/1,000 dilution. Alpaca anti-Rabbit IgG Fc secondary antibody (HA1031) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA723112 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA723112 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 18 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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