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PHOS / PDC Recombinant Rabbit Monoclonal Antibody [PSH09-54]

Usd: 385

Specification

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Catalog# HA723116

PHOS / PDC Recombinant Rabbit Monoclonal Antibody [PSH09-54]

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

PHOS / PDC Recombinant Rabbit Monoclonal Antibody [PSH09-54]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human PHOS/PDC aa 36-69/246

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IHC-Fr, IF-Tissue

Molecular Weight

Predicted band size: 28 kDa

Positive Control

Mouse eyeball tissue lysate, rat eyeball tissue lysate, mouse eyeball tissue, rat eyeball tissue.

Conjugation

unconjugated

Clone Number

PSH09-54

Reactivity Data

WB IHC-P IHC-Fr IF-Tissue
Human
Not Recommended
Mouse
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000-1:8,000

  • IHC-Fr

  • 1:500

  • IF-Tissue

  • 1:200-1:2,000

Target

Function

May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. Inhibits the transcriptional activation activity of the cone-rod homeobox CRX.

Background References

1. Jiang Z, Xiong N, Yan R, Li ST, Liu H, Mao Q, Sun Y, Shen S, Ye L, Gao P, Zhang P, Jia W, Zhang H. PDHX acetylation facilitates tumor progression by disrupting PDC assembly and activating lactylation mediated gene expression. Protein Cell. 2024 Sep 23:pwae052. doi: 10.1093/procel/pwae052. Epub ahead of print. PMID: 39311688.

2. Stacpoole PW. Therapeutic Targeting of the Pyruvate Dehydrogenase Complex/Pyruvate Dehydrogenase Kinase (PDC/PDK) Axis in Cancer. J Natl Cancer Inst. 2017 Nov 1;109(11). doi: 10.1093/jnci/djx071. PMID: 29059435.

Subcellular Location

Cytoplasm, cytosol, Nucleus, Cell projection, cilium, photoreceptor outer segment, Photoreceptor inner segment,

UNIPROT

SwissProt: P20941 Human

SwissProt: Q9QW08 Mouse

SwissProt: P20942 Rat

Synonyms

33 kDa phototransducing protein antibody

MEKA protein antibody

pdc antibody

PHD antibody

PHOS_HUMAN antibody

Phosducin antibody

Protein MEKA antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of PHOS / PDC on different lysates with Rabbit anti-PHOS / PDC antibody (<a href="/products/HA723116" style="font-weight: bold;text-decoration: underline;">HA723116</a>) at 1/2,000 dilution.<br /><br />Lane 1: mouse eyeball tissue lysate (40 µg/Lane)<br />Lane 2: mouse liver tissue lysate (negative) (40 µg/Lane)<br />Lane 3: rat eyeball tissue lysate (40 µg/Lane)<br />Lane 4: rat liver tissue lysate (negative) (40 µg/Lane)<br /><br />Predicted band size: 28 kDa<br />Observed band size: 33 kDa<br /><br />Exposure time: 3 minutes ; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723116" style="font-weight: bold;text-decoration: underline;">HA723116</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of PHOS / PDC on different lysates with Rabbit anti-PHOS / PDC antibody (HA723116) at 1/2,000 dilution.

    Lane 1: mouse eyeball tissue lysate (40 µg/Lane)
    Lane 2: mouse liver tissue lysate (negative) (40 µg/Lane)
    Lane 3: rat eyeball tissue lysate (40 µg/Lane)
    Lane 4: rat liver tissue lysate (negative) (40 µg/Lane)

    Predicted band size: 28 kDa
    Observed band size: 33 kDa

    Exposure time: 3 minutes ; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723116) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-PHOS / PDC antibody (<a href="/products/HA723116" style="font-weight: bold;text-decoration: underline;">HA723116</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723116" style="font-weight: bold;text-decoration: underline;">HA723116</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-PHOS / PDC antibody (HA723116) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723116) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat eyeball tissue with Rabbit anti-PHOS / PDC antibody (<a href="/products/HA723116" style="font-weight: bold;text-decoration: underline;">HA723116</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723116" style="font-weight: bold;text-decoration: underline;">HA723116</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat eyeball tissue with Rabbit anti-PHOS / PDC antibody (HA723116) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723116) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of frozen mouse eyeball tissue with Rabbit anti-PHOS / PDC antibody (<a href="/products/HA723116" style="font-weight: bold;text-decoration: underline;">HA723116</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span> The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA723116" style="font-weight: bold;text-decoration: underline;">HA723116</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse eyeball tissue with Rabbit anti-PHOS / PDC antibody (HA723116) at 1/500 dilution.

    The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA723116, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

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