SLC31A1 / CTR1 Recombinant Rabbit Monoclonal Antibody [PSH09-67]
Catalog# HA723132
SLC31A1 / CTR1 Recombinant Rabbit Monoclonal Antibody [PSH09-67]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SLC31A1 / CTR1 Recombinant Rabbit Monoclonal Antibody [PSH09-67]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human SLC31A1 / CTR1 aa 80-190/190
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 21 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, Caco-2 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
PSH09-67
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
High affinity copper uptake protein 1 (CTR1) is a protein that in humans is encoded by the SLC31A1 gene.Copper is an element essential for life, but excessive copper can be toxic or even lethal to the cell. Therefore, cells have developed sophisticated ways to maintain a critical copper balance, with the intake, export, and intracellular compartmentalization or buffering of copper strictly regulated. The 2 related genes ATP7A and ATP7B, responsible for the human diseases Menkes syndrome and Wilson disease, respectively, are involved in copper export. In S. cerevisiae, the copper uptake genes CTR1, CTR2, and CTR3 have been identified, and in human the CTR1 and CTR2 (MIM 603088) genes have been identified.
Background References
1. Haberkiewicz O, Lipiński P, Starzyński RR, . Decreased Expression of the Slc31a1 Gene and Cytoplasmic Relocalization of Membrane CTR1 Protein in Renal Epithelial Cells: A Potent Protective Mechanism against Copper Nephrotoxicity in a Mouse Model of Menkes Disease. Int J Mol Sci. 2022 Sep 28;23(19):11441. doi: 10.3390/ijms231911441. PMID: 36232742; PMCID: PMC9570402.
2. Jiang F, Du L, Chen ZJ, Wang X, Ge D, Liu N. LNP-miR-155 cy5 Inhibitor Regulates the Copper Transporter via the β-Catenin/TCF4/SLC31A1 Signal for Colorectal Cancer Therapy. Mol Pharm. 2023 Aug 7;20(8):4138-4152. doi: 10.1021/acs.molpharmaceut.3c00276. Epub 2023 Jun 26. Erratum in: Mol Pharm. 2023 Dec 4;20(12):6509. doi: 10.1021/acs.molpharmaceut.3c00979. PMID: 37358225.
Subcellular Location
Cell membrane ; Early endosome membrane ; Recycling endosome membrane ; Apical cell membrane ; Late endosome membrane ; Basolateral cell membrane.
Synonyms
Copper transport 1 homolog antibody
Copper transporter 1 antibody
COPT1 antibody
COPT1_HUMAN antibody
CTR1 antibody
hCTR1 antibody
High affinity copper uptake protein 1 antibody
SLC31A1 antibody
solute carrier family 31 (copper tansporters) member 1 antibody
Solute carrier family 31 member 1 antibody
Images
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Western blot analysis of SLC31A1 / CTR1 on different lysates with Rabbit anti-SLC31A1 / CTR1 antibody (HA723132) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Caco-2 cell lysate (20 µg/Lane)
Lane 4: Neuro-2a cell lysate (20 µg/Lane)
Lane 5: C2C12 cell lysate (20 µg/Lane)
Lane 6: C6 cell lysate (20 µg/Lane)
Lane 7: PC-12 cell lysate (20 µg/Lane)
Predicted band size: 21 kDa
Observed band size: 30 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723132) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"