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Nectin-4 Recombinant Rabbit Monoclonal Antibody [PSH09-73]

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Specification

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Catalog# HA723138

Nectin-4 Recombinant Rabbit Monoclonal Antibody [PSH09-73]

Application

  • WB

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Nectin-4 Recombinant Rabbit Monoclonal Antibody [PSH09-73]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Nectin-4 aa 1-400.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 55 kDa

Positive Control

MCF7 cell lysate, T-47D cell lysate, human placenta tissue, human skin tissue.

Conjugation

unconjugated

Clone Number

PSH09-73

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Not Recommended
Not Recommended
Rat
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Nectins and Nectin-like molecules (Necl) are families of cellular adhesion molecules involved in Ca2+-independent cellular adhesion. Nectins are ubiquitously expressed and have adhesive roles in a wide range of tissues such as the adherens junction of epithelia or the chemical synapse of the neuronal tissue. So far four nectins have been identified in humans, namely nectin-1, nectin-2, nectin-3 and nectin-4. These four family members have also been found in most other well studied mammals. Also, five Necls have been identified, these are: Necl-1, Necl-2, Necl-3, Necl-4 and Necl-5.

Background References

1. Chatterjee S et al. Nectin cell adhesion molecule-4 (NECTIN-4): A potential target for cancer therapy. Eur J Pharmacol. 2021 Nov

2. Bouleftour W et al. Nectin-4: a Tumor Cell Target and Status of Inhibitor Development. Curr Oncol Rep. 2023 Mar

Subcellular Location

Cell membrane, Cell junction, adherens junction, Secreted.

UNIPROT

SwissProt: Q96NY8 Human

Synonyms

DKFZp686K05193 antibody

EDSS1 antibody

Ig superfamily receptor LNIR antibody

Nectin 4 antibody

Nectin-4 antibody

poliovirus receptor-related 4 antibody

Processed poliovirus receptor-related protein 4 antibody

PRR4 antibody

pvrl4 antibody

PVRL4_HUMAN antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Nectin-4 on different lysates with Rabbit anti-Nectin-4 antibody (<a href="/products/HA723138" style="font-weight: bold;text-decoration: underline;">HA723138</a>) at 1/2,000 dilution.<br /><br />Lane 1: MCF7 cell lysate (15 µg/Lane)<br />Lane 2: A549 cell lysate (negative) (15 µg/Lane)<br />Lane 3: MDA-MB-231 cell lysate (negative) (15 µg/Lane)<br />Lane 4: T-47D cell lysate (15 µg/Lane)<br /><br />Predicted band size: 55 kDa<br />Observed band size: 45-70 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723138" style="font-weight: bold;text-decoration: underline;">HA723138</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Nectin-4 on different lysates with Rabbit anti-Nectin-4 antibody (HA723138) at 1/2,000 dilution.

    Lane 1: MCF7 cell lysate (15 µg/Lane)
    Lane 2: A549 cell lysate (negative) (15 µg/Lane)
    Lane 3: MDA-MB-231 cell lysate (negative) (15 µg/Lane)
    Lane 4: T-47D cell lysate (15 µg/Lane)

    Predicted band size: 55 kDa
    Observed band size: 45-70 kDa

    Exposure time: 42 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723138) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Nectin-4 antibody (<a href="/products/HA723138" style="font-weight: bold;text-decoration: underline;">HA723138</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723138" style="font-weight: bold;text-decoration: underline;">HA723138</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Nectin-4 antibody (HA723138) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723138) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Nectin-4 antibody (<a href="/products/HA723138" style="font-weight: bold;text-decoration: underline;">HA723138</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723138" style="font-weight: bold;text-decoration: underline;">HA723138</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Nectin-4 antibody (HA723138) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723138) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation